目的 应用高内涵筛选技术（high content screening，HCS）对硫熏麦冬的肝毒性进行研究。方法 以人肝癌细胞系HepG2细胞为载体，分别设置阳性对照药（噻氯吡啶）、阴性对照药（阿司匹林）、麦冬提取物、硫熏麦冬提取物组，利用HCS技术测定并分析不同药物的细胞数目、DNA含量、谷胱甘肽（GSH）降低水平、活性氧（ROS）含量和线粒体膜电位（MMP）。结果 与麦冬提取物组细胞相比，硫熏麦冬提取物组从50 mg/mL开始，GSH降低水平超过肝毒性的阈值；从12.5 mg/mL开始，硫熏麦冬提取物组细胞MMP的改变也超过毒性阈值。结论 麦冬硫熏后表现出潜在的肝毒性，由其对MMP的影响推测硫熏致肝毒性可能与线粒体介导的细胞凋亡有关。

Objective An efficient method was established using high content screening (HCS) for the hepatotoxicity evaluation of sulfur-fumigated Ophiopogonis Radix. Methods Cytotoxicity of positive control group, negative control group, Ophiopogonis Radix extracts group and sulfur-fumigated Ophiopogonis Radix extracts group were tested based on HepG2 human hepatoma cells. HCS was applied to detect the cell number, DNA content, level of glutathione (GSH), reactive oxgyen species (ROS), and mitochondrial membrane potential (MMP). Results Compared with the cells of Ophiopogonis Radix extracts group, GSH of sulfur-fumigated Ophiopogonis Radix extracts decreased significantly at the concentration of 50 mg/mL; The MMP of sulfur-fumigated Ophiopogonis Radix extracts changed signally at the concentration of 12.5 mg/mL. Conclusion Ophiopogonis Radix showed pontential cytotoxicity after sulfur-fumigated. The hepatotoxicity of sulfur-fumigated Ophiopogonis Radix may be related to mitochondria-mediated apoptosis according to the influence of its MMP from the results.

2017年北京市科技新星与领军人才培养专项（Z171100001117029）；2018北京中医药大学杰出青年人才项目（2018-JYB-XJQ008）；2019北京中医药大学创新创业项目（2019-JYB-XSCXCY-06）