[关键词]
[摘要]
目的 基于HPLC-Q/TOF-MS系统解析保阴煎化学物质组。方法 采用Diamonsil C18(250 mm×4.6 mm,5 μm)色谱柱,以乙腈-0.1%甲酸水溶液进行梯度洗脱,柱温30℃,体积流量1.0 mL/min,进样量10 μL,质谱条件为X500R QTOF质谱仪,电喷雾离子源,正、负离子模式扫描。结果 通过对照品确认、高分辨质谱数据分析及文献比对,共鉴别出52个化学成分,包括17个黄酮类、6个酚酸类、12个环烯醚萜类、8个生物碱类、1个苯乙醇苷类、4个单萜类、2个三萜类及2个其他类成分。结论 建立的方法可系统、准确、快速地鉴定保阴煎中的多种化学成分,为后续保阴煎质量属性的确定提供了依据。
[Key word]
[Abstract]
Objective To analyze the chemical constituent cluster of classical herbal formulae Baoyinjian systemically by HPLC-Q/TOF-MS. Methods The seperation was performed on Diamonsil C18 (250 mm×4.6 mm, 5 μm) column with gradient elution with acetonitrile-0.1% formic acid. The column temperature was 30℃, the flow rate was 1 mL/min, the injection volume was 10 μL, and the mass spectrometry condition was X500R QTOF mass spectrometer, electrospray ion source, positive and negative mode scanning. Results A total of 52 chemical constituents were identified by reference confirmation, literature comparison, and high mass spectrometry data analysis. The chemical constituent cluster was composed of 17 flavonoids, six phenolics, 12 iridoid glycosides, eight alkaloids, one phenethyl alcohol glycosides, four monoterpene glycoside, two triterpenes and two other compound. Conclusion This study can identify various chemical constituents of Baoyinjian systematically, accurately, and rapidly, which provides a basis for the determination of the quality attributes of Baoyinjian.
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[基金项目]
国家自然科学基金重点资助项目(81430096)