[关键词]
[摘要]
目的 优选三七含量测定时供试品溶液的制备方法。方法 采用HPLC法同时测定三七皂苷R1、人参皂苷Rg1、人参皂苷Rb1,结合Box-Behnken设计-响应面法考察提取溶剂、料液比、提取温度对皂苷类成分提取工艺的影响,优选供试品溶液的制备方法。结果 最佳制备方法为采用75%甲醇,回流提取1次,料液比为1:40,提取温度为100℃。结论 优选出的供试品溶液制备方法简单可行,成分提取率较高,为三七含量测定时供试品溶液制备提供参考。
[Key word]
[Abstract]
Objective To study and optimize the preparation method of test solution from Panax notoginseng. Methods The effects of extraction solvent, liquid-material ratio and extraction temperature on the optimization of the preparation method of test solution of notoginsenoside and ginsenosides in P. notoginseng were investigated by Box-Behnken response surface methology. The content of notoginsenoside R1, ginsenoside Rg1, and ginsenoside Rb1 was simultaneously determined by HPLC. Results The optimum method was as follow:75% methanol was used for once reflux extraction, the ratio of liquid-material ratio was 1:40, the extraction temperature was 100℃. Conclusion The optimum preparation of test solution is simple and feasible, and the extraction rate of components is high, which provides a reference for the preparation of test solution from P. notoginseng.
[中图分类号]
[基金项目]