目的 制备马钱子总碱（TASS）-白芍总苷（TGP）脂质立方液晶纳米粒（TASS-TGP LLCN），并考察其体外经皮吸收行为。方法 运用前体注入法制备TASS-TGP LLCN，采用超滤离心法测定包封率，以包封率为指标采用均匀设计优化TASS-TGP LLCN处方，并对优化后制备的TASS-TGP LLCN基本性能进行评价。同时，采用泊洛沙姆407（F127）作为凝胶基质制备凝胶，运用Franz扩散池法比较TASS-TGP LLCN凝胶和TASS-TGP普通凝胶的体外经皮渗透特性，初步考察TASS-TGP LLCN凝胶的经皮渗透行为。结果 TASS-TGP LLCN的最佳制备处方为甘油单油酸酯（GMO）1.0 g，F127 0.25 g，分散相60 mL，马钱子碱、士的宁及芍药苷包封率均大于50%，制剂质量评价显示制得的纳米粒平均粒径为（245.3±16.4）nm，pH值6.62，在透射电镜下呈立方体结构，大小均一；透皮实验显示，TASS-TGP LLCN凝胶的24 h累积透过量、渗透速率及皮肤滞留量均大于TASS-TGP普通凝胶。结论 TASS-TGP LLCN呈现促渗和皮肤贮库的双重效应，具有潜在的开发前景。

Objective To prepare total alkaloids of Strychni Semen (TASS)-total glucosides of paeony (TGP) lipid-based cubic liquid crystalline nanoparticles (TASS-TGP LLCN) and investigate its percutaneous absorption behavior. Methods TASS-TGP LLCN was prepared by precursor injection method, and the encapsulation efficiency (EE) was determined by ultrafiltration centrifugation. The prescription of TASS-TGP LLCN was optimized by uniform design with the encapsulation efficiency as the index, and the basic properties of the optimized TASS-TGP LLCN were evaluated. Meanwhile, Poloxamer 407 (F127) was used as matrix to prepare gel, Franz diffusion method was used to compare the in vitro percutaneous permeability of TASS-TGP LLCN gel with TASS-TGP ordinary gel. Results The optimal formula of TASS-TGP LLCN was glycerol monooleate (GMO) 1.0 g, F127 0.25 g, dispersed phase 60 mL. The EE of brucine, strychnine and paeoniflorin were all more than 50%, the average particle size was about (245.3 ±16.4) nm, the pH value was 6.62, and the cubic structure was uniform in size under transmission electron microscope. The cumulate osmotic quantities in 24 h, the permeation rate and the skin retention volume of TASS-TGP LLCN gel were all better than ordinary gel of TASS-TGP. Conclusion TASS-TGP LLCN has dual effects of promoting permeability and skin reservoir, which has a potential development prospect.

国家自然科学基金资助项目（81760717）；国家自然科学基金资助项目（81460606）；江西省教育厅科学技术研究项目（GJJ171541）