目的 建立补骨脂中16个化学成分的一测多评分析方法，结合化学计量学评价不同批次补骨脂药材的质量差异。方法 采用高效液相色谱法，以异补骨脂素为内参物，测得待测成分的相对校正因子，在不同色谱仪和色谱柱上考察每个化学成分的相对校正因子的稳定性和耐用性；比较一测多评法和外标法测定结果的差异，以此验证一测多评法在补骨脂中应用的准确性。结果 在建立的色谱条件下，补骨脂中15种化学成分相对于异补骨脂素的相对校正因子准确性较高，耐用性较好，且在不同实验条件下重现性良好；2种分析方法所得结果无显著差异。不同批次间补骨脂的化学成分群一致性较好，但不同化学成分的含量差异较大。结论 新建立的同时测定补骨脂中16个化学成分的一测多评法，能为更加全面地评价不同来源补骨脂的整体质量提供参考。

Objective The purpose of this study was to establish a QAMS analytical method for 16 compounds in Psoraleae Fructus, and attempt to evaluate the quality difference among different batches of Psoralen Fructus by chemometrics. Methods All experiments were performed on three different HPLC instruments. Isopsoralen was used as the internal reference substance to determine the relative correction factors of the other 15 compounds. The robustness and durability of the measured relative correction factors of the 15 compounds were evaluated on different chromatograph instruments and columns; And the measurement result deviation was compared between QAMS method and the external standard method. Results Under the established chromatographic conditions, the relative correction factors of 15 compounds in Psoraleae Fructus had high accuracy, good durability, and good reproducibility under different experimental conditions. The results obtained from two analysis methods showed no significant deviation. The results obtained by the new established QAMS analytical method showed that the consistency of compound types among different batches of Psoraleae Fructus were better, but the content of different componounds was relatively different. Conclusion The newly established QAMS analytical method for simultaneous determination of 16 compounds in Psoraleae Fructus provides a more efficient method to evaluate the comprehensive quality of Psoraleae Fructus from different sources.

国家重点研发计划（2018YFC1707000）；四川省科技厅项目（2019YJ0640）；成都市科技局项目（2016-HM01-00312-SF）