[关键词]
[摘要]
目的 建立黄连解毒汤(HJD)中13个活性成分黄柏碱、绿原酸、木兰花碱、京尼平苷、黄连碱、表小檗碱、药根碱、小檗碱、巴马汀、黄芩苷、汉黄芩苷、汉黄芩素、千层纸素A的HPLC检测方法,利用活性成分含量和比例筛选HJD有效部位。方法 借助索氏提取器采用系统溶剂提取法将HJD水煎液冻干粉依次经石油醚、醋酸乙酯、正丁醇、纯净水提取并干燥后获得HJD不同极性部位,将残渣干燥后作为沉淀部位。采用Agilent 1260型高效液相色谱仪建立HJD中13种活性成分的HPLC检测方法,并对HJD及其不同极性部位中活性成分含量进行检测,筛选HJD有效部位。结果 成功建立了稳定可靠的HPLC检测方法;HJD中各活性成分含量从高到低依次为京尼平苷、小檗碱、巴马汀、黄芩苷、汉黄芩苷、黄连碱、药根碱、表小檗碱、木兰花碱、黄柏碱、汉黄芩素、绿原酸、千层纸素A。与石油醚、醋酸乙酯、水和沉淀部位相比,HJD正丁醇部位中各活性成分总量最高且其比例与HJD全方相近,为HJD有效部位。结论 成功建立了HJD中13个活性成分的HPLC检测方法,经筛选HJD正丁醇部位为HJD有效部位。
[Key word]
[Abstract]
Objective To establish a method for the determination of 13 active components (phellodendrine, chlorogenic acid, magnoflorine, geniposide, coptisine, epiberberine, jatrorrhizine, berberine, palmatine, baicalin, wogonoside, wogonin, and oroxylin A) in Huanglian Jiedu Decoction (HJD) by HPLC, and screen the effective fractions of HJD by the content and ratio of active components. Methods The freeze-dried powder of HJD water decoction was extracted by petroleum ether, ethyl acetate, n-butanol and pure water by soxhlet extractor combined system solvent extraction method. The different polar fractions of HJD were obtained after drying, and the residue was used as the precipitation fraction. Subsequently, 13 active ingredients in HJD were detected by Agilent 1260 high performance liquid chromatography. Furthermore, the content of active components in HJD and its different polar fractions were detected, and the effective fraction of HJD were screened. Results A stable and reliable HPLC method was successfully established, and the content of active components in HJD from high to low were:geniposide, berberine, palmatine, baicalin, wogonoside, coptisine, jatrorrhizine, epiberberine, magnoflorine, phellodendrine, wogonin, chlorogenic acid, and oroxylin A. Compared with the fractions of petroleum ether, ethyl acetate, purified water, and precipitation, the total content of active components in the fraction of n-butanol of HJD was the highest and its proportion is the closest to that of HJD, which was the effective fraction of HJD. Conclusion In this study, a method for the determination of 13 active ingredients in HJD by HPLC was successfully established, and the n-butanol fraction of HJD was selected as the effective fraction of HJD based on the content of active components.
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[基金项目]
国家肉牛/牦牛产业技术体系(CARS-37);甘肃农业大学优秀博士学位论文培育项目(YB2017004)