[关键词]
[摘要]
目的 研究板蓝根Radix isatidis中的糖苷类化学成分。方法 采用硅胶、反相ODS C18、葡聚糖凝胶等柱色谱法、半制备液相色谱法等色谱技术分离纯化,NMR、MS、IR等波谱学技术解析结构。结果 从板蓝根中共分离得到了12个糖苷类化合物,分别鉴定为菘蓝苷C(1)、表原告依春(2)、原告依春(3)、o-aminobenzoic acid-7-O-β-D-glucopyranosyl ester(4)、2'-O-methyladenosine(5)、2-methoxyphenyl-β-D-glucopyranoside(6)、松柏苷(7)、丁香苷(8)、顺式松柏苷(9)、顺式丁香苷(10)、corchoionoside C(11)和麻叶千里光苷D(12)。结论 化合物1为新化合物,化合物5~7、9~12为首次从板蓝根中分离得到。
[Key word]
[Abstract]
Objective To study the glycosides from the roots of Isatis indigotica. Methods The chemical components were isolated and purified by silica gel, Sephadex LH-20, ODS C18 gel, and semi preparative-HPLC chromatographic techniques while the structures were deduced by NMR, MS and IR spectral data analysis. Results A total of 12 glycosides were obtained and elucidated as isatindigoside C (1), epiprogoitrin (2), progoitrin (3), o-aminobenzoic acid 7-O-β-D-glucopyranosyl ester (4), 2'-O-methyladenosine (5), 2-methoxyphenyl β-D-glucopyranoside (6), coniferin (7), syringin (8), cis-coniferin (9), cis-syringin (10), corchoionoside C (11), and cannabiside D (12). Conclusion Compound 1 is a new indole alkaloid glycoside which named as isatindigoside C, while compounds 5-7 and 9-12 are isolated from the plant for the first time.
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[基金项目]
国家自然科学基金资助项目(81573571);国家自然科学基金资助项目(81673570);上海市优秀学术带头人计划项目(16XD1403500);上海市“中药药效物质”E-研究院项目;上海市科技创新行动计划项目(18401931100)