[关键词]
[摘要]
目的 明确疏风解毒胶囊发挥疏风解表作用的潜在物质基础。方法 利用LC-MS建立疏风解毒胶囊22个不同药材配比样品的MS谱库;采用与疏风解表相关的M3型乙酰胆碱受体高表达的中国仓鼠卵巢癌细胞CHO-K1/M3为体外药效筛选模型,检测22个样品对M3受体的激动活性;以22个样品特征峰的相对峰面积为自变量,以对M3受体的激动率为因变量,采用BP-神经网络算法进行谱-效关联分析。结果 通过建立的最佳神经网络模型,计算得到16个特征峰与疏风解毒胶囊对M3受体的激动活性显著相关。结论 通过谱效研究,推测疏风解毒胶囊发挥疏风解表的药效物质基础可能为包括二氢败酱苷、大黄素、大黄酸、连翘苷、连翘酯苷E、柴胡皂苷d、虎杖苷、毛蕊花糖苷等在内的16个化合物,为其质量标志物的确定提供了依据。
[Key word]
[Abstract]
Objective To explore the potential effective compounds of ‘wind-dispersing and exterior syndrome-relieving’ of Shufeng Jiedu Capsule (SJC). Methods The fingerprints of 22 samples with different proportions of SJC were established by LC-MS. The stimulation rates of 22 samples to the M3 receptor were measured by Chinese hamster ovarian cancer cells with high expression of M3 acetylcholine receptor which related to ‘wind-dispersing and exterior syndrome-relieving’. Finally, the spectrum-effect relationship of relative peak areas of characteristic peaks and stimulation rates was analyzed by BP-neural network. Results According to the established optimal neural network model, 16 characteristic peaks were calculated and found to be significantly correlated with the activity of SJC on the M3 receptor stimulation. Conclusion Through the spectrum-effect study, it is speculated that the effective components of SJC on ‘wind-dispersing and exterior syndrome-relieving’ may be the 16 compounds including dihydrodylate glycoside, emodin, rhein, phillyrin, forsythoside E, saikosaponin d, polydatin, verbascoside, etc, which provides a basis for the determination of quality markers.
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[基金项目]
国家中药标准化专项资助项目(ZYBZH-C-AH-02);国家自然科学基金资助项目(81430096);国家自然科学基金资助项目(81703802)