目的 建立不同批次金花葵的多波长HPLC指纹图谱，并结合定量分析、相似度评价、聚类分析及主成分分析对金花葵进行质量评价研究。方法 采用Phenomenex Kinetex C₁₈（250 mm×4.6 mm，5 μm）色谱柱，以乙腈-0.08%磷酸水为流动相，梯度洗脱，体积流量为1.0 mL/min，波长切换（0~8 min，260 nm，检测原儿茶酸；8~15 min，324 nm，检测咖啡酸；15~60 min，360 nm，检测芦丁、金丝桃苷、异槲皮苷、棉皮素-8-O-β-D-葡萄糖醛酸苷、杨梅素、槲皮素-3'-O-葡萄糖苷、槲皮素），柱温为30℃。利用中药色谱指纹图谱相似度评价系统2004A版及SPSS 19.0软件建立金花葵HPLC指纹图谱，并进行相似度评价、聚类分析及主成分分析。结果 金花葵HPLC指纹图谱确认了25个共有峰，指认9个共有峰，并对指认共有峰进行了含量测定。16批金花葵样品相似度在0.879~0.983，相似度良好；聚类分析结果将金花葵聚为2类，表明不同产地金花葵在相似中存在差异性；根据主成分综合得分对金花葵质量进行了排名。结论 该方法简便准确，可用于金花葵药材的综合质量评价。

Objective To establish multiwavelength HPLC fingerprint of Aurea helianthus from different batches, and combine quantitative analysis, similarity evaluation, cluster analysis, and principal component analysis to evaluate the quality of A. helianthus. Methods The chromatographic column was Phenomenex Kinetex C₁₈ (250 mm×4.6 mm, 5 μm). The mobile phase was composed of 0.08% phosphoric acid water (A) and acetonitrile (B) in gradient elution at a flow rate of 1.0 mL/min, the detection wavelength was set at 260 nm for protocatechuic acid during 0-8 min, 324 nm for caffeic acid during 8-15 min, 360 nm for rutin, hyperin, isoquercitrin, gossypetin-8-O-β-D-glucuronide, myricetin, quercetin-3'-O-glucoside, and quercetin during 15-60 min. The column temperature was set at 30℃. And the HPLC fingerprint of A. helianthus was established by the similarity evaluation system for chromatographic fingerprint of TCM (Version 2004A) and SPSS19.0, which was used for similarity evaluation, cluster analysis, and principal component analysis. Results A total of 25 common peaks were confirmed of A. helianthus HPLC fingerprint, and nine peaks were identified which were determined. The similarity of 16 batches of samples was between 0.879 and 0.983; The results of cluster analysis showed that A. helianthus was clustered into two groups, indicating that there were differences in the similarity; Ranked the quality of A. helianthus based on the main component composite score. Conclusion The method is simple and accurate, which can be used for the comprehensive quality evaluation research of the medicinal materials of A. helianthus.

R286.2

河南省基础与前沿技术研究项目（112300410063）