目的 探讨丹参茎叶提取物及其主要成分对氧化应激和高糖损伤的人脐静脉内皮细胞（HUVECs）的保护作用及机制。方法 建立HUVECs氧化应激与高糖损伤模型，分别给予不同剂量的丹参茎叶醇提物（CJ）、丹参茎叶水提物（SJ）、丹参根醇提物（CG）、丹参根水提物（SG）及迷迭香酸、丹酚酸B、芦丁、异槲皮苷、隐丹参酮等，MTT法检测细胞活力，检测谷胱甘肽过氧化物酶（GSH-Px）、过氧化氢酶（CAT）、一氧化氮（NO）、内皮素（ET-1）、细胞黏附因子（ICAM-1）和肿瘤坏死因子-α（TNF-α）的表达水平。结果 与对照组比较，HUVECs经H₂O₂处理后，GSH-Px、CAT和NO含量降低（P<0.01），ET-1含量升高（P<0.01）；HUVECs经高糖处理后，ICAM-1和TNF-α含量升高（P<0.01），NO含量降低（P<0.01）。与模型组比较，CJ、CG、SJ、SG组GSH-Px、CAT和NO含量升高，ET-1、ICAM-1、TNF-α含量降低（P<0.05、0.01）。迷迭香酸、丹酚酸B、芦丁等的高、中剂量组，异槲皮苷、隐丹参酮高剂量组均能显著提高GSH-Px、CAT和NO含量（P<0.05、0.01），降低ET-1含量（P<0.05、0.01），丹酚酸B、异槲皮苷和芦丁的高剂量组及迷迭香酸的高、中剂量组均可显著降低ICAM-1含量（P<0.01），除氨基胍给药组外，其他给药组TNF-α含量相比模型组明显降低（P<0.05、0.01）。除氨基胍和异槲皮苷低剂量组，其他给药组NO含量均明显升高（P<0.05、0.01）。结论 一定质量浓度范围内的丹参茎叶与丹参根醇提物及水提物、迷迭香酸、丹酚酸B、芦丁、异槲皮苷对H₂O₂和高糖引起的HUVECs细胞损伤具有明显保护作用，其作用机制与抑制细胞间黏附分子的表达，调节NO和TNF-α生成密切相关。本研究将为丹参药材生产过程产生的非药用部位茎叶的资源价值发现及转化利用提供参考。

Objective To investigate the protective effects of the extracts and active components from stems and leaves of Salvia miltiorrhiza on oxidative stress and high glucose-injured human umbilical endothelial cells (HUVECs). Methods The models of oxidative stress and high glucose injury in HUVECs were established. The ethanol extract of S. miltiorrhiza stems and leaves (CJ), ethanol extract of S. miltiorrhiza roots (CG), water extract of S. miltiorrhiza stems and leaves (SJ), water extract of S. miltiorrhiza roots (SG), rosmarinic acid, salvianolic acid B, rutin, isoquercitrin, cryptotanshinone, aminoguanidine and VC were administrated to cells. MTT were used to observe the cell viability. The levels of glutathione peroxidase (GSH-Px), catalase (CAT), nitric oxide (NO), endothelin (ET-1), ICAM-1 and TNF-α were detected. Results Compared with the control group, H₂O₂ decreased the levels of GSH-Px, CAT and NO (P < 0.01) and increased the level of ET-1 (P < 0.01), glucose increased the levels of ICAM-1 and TNF-α (P < 0.01) and decreased the level of NO (P < 0.01). Compared with the model group, the levels of GSH-Px, CAT and NO in CJ, CG, SJ and SG groups were increased, and the levels of ET-1, ICAM-1 and TNF-α were decreased (P < 0.05, P < 0.01). VC, rosmarinic acid, salvianolic acid B and rutin high and medium dose groups, and isoquercitrin, cryptotanshinone high dose group significantly increased the levels of GSH-Px, CAT and NO (P < 0.05, P < 0.01) and decreased the level of ET-1 (P < 0.05, P < 0.01). ICAM-1 levels were significantly decreased in the high dose groups of salvianolic acid B, isoquercitrin and rutin as well as in the high and medium dose groups of rosmarinic acid (P < 0.01). In addition to the aminoguanidine group, the levels of TNF-α of other groups were significantly lower than the model group (P < 0.05, P < 0.01). Except the aminoguanidine group and isoquercetin low dose group, the levels of NO of other groups were significantly higher than the model group (P < 0.05, P < 0.01). Conclusion In certain concentration range of alcohol extract and water extract of stems, leaves and roots of S. miltiorrhiza, rosmarinic acid, salvianolic acid B, rutin and isoquercitrin have protective effects on HUVECs injured by H₂O₂ and glucose. And the mechanisms are related to inhibition of intercellular adhesion molecule expression and regulation of NO and TNF-α production. This study will provide reference for the discovery and transformation of the resource value of non-medicinal stems and leaves produced during the production of S. miltiorrhiza.

R285.5