[关键词]
[摘要]
目的 建立一种对中药制剂前列欣胶囊中复杂成分快速、高效的鉴定分析方法,为系统阐述其化学组成提供基础研究数据。方法 采用高效液相色谱-四级杆/静电场轨道阱高分辨质谱(UHPLC-Q-Orbitrap HRMS)对前列欣胶囊中的化学成分进行鉴定分析。通过多级碎片离子信息与对照品比对,结合文献数据对化合物进行准确定性。结果 共鉴定出前列欣胶囊中61种化学成分,主要包括黄酮类12种、香豆素类11种、萜类6种、有机酸类19种及其他类。结论 本方法可快速、准确鉴定前列欣胶囊中的多种化学成分,为其物质基础和质量控制研究提供理论依据。
[Key word]
[Abstract]
Objective In order to establish an efficient analysis method for the identification of the complex components in Qianliexin Capsule rapidly, and provide the basic research data for the systematic elaboration of its chemical constituents. Methods An ultra-high performance liquid chromatography-quadrupole/Orbitrap high resolution mass spectrometry (UHPLC-Q-Orbitrap HRMS) was used for the identification analysis of the components in Qianliexin Capsule accurately, and the multistage fragments ions data was compared with the standard substance and consulting the literatures. Results Sixty-one compounds were identified in this study, including 12 flavones, 11 coumarins, six terpenes, 19 organic acids and other categories. Conclusion The accurate and rapid identification of various chemical constituents of Qianliexin Capsule was achieved in this study, which provided the theory basis for the pharmacodynamic material and the quality control study of Qianliexin Capsule.
[中图分类号]
R284.1
[基金项目]
郑州大学第一附属医院青年创新基金项目(2014)