[关键词]
[摘要]
目的 研究杜仲Eucommia ulmoides皮的化学成分。方法 采用正相硅胶柱色谱、反向ODS柱色谱、Sephadex LH-20柱色谱、制备HPLC等手段对杜仲皮化学成分进行分离纯化,并根据HR-ESI-MS、NMR等波谱数据进行结构鉴定。结果 从杜仲皮95%乙醇提取物中分离得到9个化合物,鉴定为甘草黄酮B(1)、3,5,4'-三羟基-7,3'-二甲氧基黄酮(2)、甘草素(3)、formononetine(4)、(+)-4'-O-methylglabridin(5)、paratocarpin E(6)、龙血素C(7)、threo-1-(4-hydroxy-3-methoxyphenyl)-1-methoxy-2-{4-[1-formyl-(E)-vinyl]-2-methoxyphenoxy}-3-propanol(8)、diospyrosin(9),其中7个黄酮和2个苯丙素类。结论 化合物1~9均首次从杜仲属植物中分离得到。
[Key word]
[Abstract]
Objective To study the chemical constituents of barks of Eucommia ulmoides. Methods Compounds were isolated by means of normal phase silical gel, reverse phase ODS, Sephadex LH-20 column chromatography, and preparative-HPLC, and their structures were identified on the basis of various spectroscopic analyses, including NMR and HR-ESI-MS. Results Nine known compounds, including seven flavonoids and two phenylpropanoids, were isolated from the 95% EtOH extract of barks of E. ulmoides. Their structures were identified as licoflavone B (1), 3,5,4'-trihydroxy-7,3'-dimethoxyflavone (2), liquiritigenin (3), formononetine (4), (+)-4'-O-methylglabridin (5), paratocarpin E (6), loureirin C (7), threo-1-(4-hydroxy-3-methoxyphenyl)-1-methoxy-2-{4-[1-formyl-(E)-vinyl]-2-methoxyphenoxy}-3-propanol (8), and diospyrosin (9). Conclusion Compounds 1-9 are obtained from the genus of Eucommia for the first time.
[中图分类号]
R284.1
[基金项目]
国家自然科学基金重点项目(81430095);国家自然科学青年基金资助项目(81303180)