[关键词]
[摘要]
目的 研究萝藦荚(萝藦Metaplexis japonica干燥果壳)的化学成分。方法 采用硅胶柱色谱、凝胶色谱、反相中压液相、制备HPLC等色谱技术进行分离纯化,根据所得化合物的理化性质和波谱数据鉴定其结构。结果 从萝藦荚95%乙醇提取物中分离得到10个化合物,分别鉴定为(15β,21α)-二羟基-孕甾-17,20-氧杂环丁烷-4,6-二烯-3-酮(1)、25-hydroperoxycyc-loart-23-en-3β-ol(2)、α-香树脂醇(3)、滨蒿内酯(4)、aleuritin(5)、丁香脂素(6)、夜来香素3-O-β-D-吡喃夹竹桃苷(7)、(20β)-21-dihydroxypregna-4,6-dien-3-one(8)、blumenol A(9)、4-pregnen-20,21-diol-3-one(10)。结论 化合物1为1个新的C21甾体类化合物,命名为新萝藦苷元F,化合物2、10为首次从萝藦科萝藦属植物中分离得到,化合物8为首次从该植物中分离得到。
[Key word]
[Abstract]
Objective To study the chemical constituents from the pericarpium of Metaplexis japonica. Methods The petroleum ether, dichloromethane and ethyl acetate fractions of 95% ethanol extract from M. japonica were isolated and purified by silica gel, Sephadex LH-20, MPLC and preparative HPLC, etc. The structures of the obtained compounds were identified by physicochemical properties and spectral data. Results Ten compounds were isolated and identified as (15β,21α)-dihydroxypregna-17,20-trimethylene oxide-4,6-dien-3-one (1), 25-hydroperoxycyc-loart-23-en-3β-ol (2), α-amyrin (3), scoparone (4), aleuritin (5), syringaresinol (6), pergularin-3-O-β-D-oleanodropyranose (7), (20β)-21-dihydroxypregna-4,6-dien-3-one (8), blumenol A (9) and 4-pregnen-20,21-diol-3-one (10). Conclusion Compound 1 is a new C21 steroidal compound named metajapogenin F, compounds 2 and 10 are isolated from the genus Metaplexis for the first time, and compound 8 is isolated from this plant for the first time.
[中图分类号]
R284.1
[基金项目]
国家自然科学基金资助项目(81573577)