[关键词]
[摘要]
目的 采用LC-MS建立绞股蓝药材的指纹图谱,鉴定其主要色谱峰,并结合变量模式识别方法判别不同产地绞股蓝药材。方法 采用Inertsil ODS-SP(250 mm×4.6 mm,5 μm)C18色谱柱,以乙腈-0.1%甲酸水为流动相梯度洗脱,检测波长203 nm,使用电喷雾离子源(ESI),负离子模式下采集数据。通过聚类分析、主成分分析和对应分析对21批绞股蓝药材进行分析。结果 建立了绞股蓝药材指纹图谱,标定出9个共有峰与10个特征峰,并根据质谱信息及文献对照对其中16个色谱峰进行定性分析。并结合多变量分析区分了不同产地绞股蓝,为绞股蓝的质量控制及综合评价奠定了基础。结论 液质联用-模式识别方法可用于不同产地绞股蓝的快速鉴别,也可对其特征成分进行靶向分离。
[Key word]
[Abstract]
Objective The fingerprint of Gynostemma pentaphyllum was established with liquid chromatograph-mass spectrometer (LC-MS), and the main chromatographic peaks were preliminary identified, and combined with multi-variable analysis pattern recognition method to identify G. pentaphyllum from different origins. Methods Inertsil ODS-SP (250 mm×4.6 mm, 5 μm) C18 column was eluted with acetonitrile-0.1% formic acid as mobile phase gradient. The detection wavelength was 203 nm. The mass spectrometry equipped with electrospray ionization (ESI) was used as detector and operated under the negative ion mode. A total of 21 batches of G. pentaphyllum were analyzed by cluster analysis, principal component analysis, and corresponding analysis. Results The fingerprints of G. pentaphyllum were established by LC-MS, and nine common peaks and 10 characteristic peaks were calibrated. According to the mass spectrometry information and literature comparison, 16 chromatographic peaks were qualitatively analyzed. Combined with multivariate analysis, G. pentaphyllum from different habitats was distinguished. It would establish the foundations for quality control and comprehensive evaluation of G. pentaphyllum. Conclusion The LC-MS and pattern recognition analysis can be used to distinguish G. pentaphyllum from different regions and the targeting compounds of interest would be separated.
[中图分类号]
[基金项目]
国家自然科学基金资助项目(81673692);中央民族大学研究生自主科研项目(SSZZKY-2019110);国家重点研发计划资助项目(2017YFC1704000)