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[摘要]
目的 考察不同时期刺五加叶中8种有效成分含量和干质量的动态变化规律,探讨刺五加叶的适宜采收期。方法 采集不同时期刺五加叶片,用电子天平测定百叶干质量;采用HPLC对不同时期刺五加叶中L-苯丙氨酸、原儿茶酸、紫丁香苷、绿原酸、咖啡酸、芦丁、金丝桃苷和槲皮素进行测定,计算8种有效成分积累总量,并与主成分分析法相结合进行综合评价。结果 不同时期刺五加的百叶干质量在S1~S5期间整体呈上升趋势,在S1~S2增长最为迅速;不同时期刺五加叶中8种有效成分的含量呈动态变化,原儿茶酸在S1(6月3日)期含量达到最大值;其他7种成分均在S2(7月3日)期含量达到最大值,且主成分分析结果也显示于S2期采收的刺五加叶中8种有效成分含量的综合得分最高;8种有效成分在不同时期的积累总量呈先上升后下降的变化规律,在S1~S2期间总量总体呈上升趋势,S2时达到最大。结论 综合不同时期刺五加叶的干质量和8种有效成分含量的变化可知,S2期前后(6月下旬至7月上旬)为最适宜采收期。为确定刺五加叶的适宜采收期提供基础资料。
[Key word]
[Abstract]
Objective The dynamic changes of eight active constituents and dry weight of Acanthopanax senticosus leaves in different periods were investigated, and the suitable harvesting period of A. senticosus leaves was discussed. Methods The leaves of A. senticosus were collected at different times, and the dry weight of one hundred leaves was determined by electronic balance. The contents of L-phenylalanine, protocatechuic acid, syringin, chlorogenic acid, caffeic acid, rutin, hyperoside, and quercetin in the leaves of A. senticosus in different periods were determined by HPLC, and the total amount of accumulation of the eight active ingredients was calculated and combined with the analysis results of the principal component analysis method for comprehensive evaluation. Results The results showed that the vegetative dry weight of A. senticosus increased during the period from S1 to S5, and increased most rapidly in S1—S2. The content of eight active constituents in the leaves of A. senticosus showed dynamic changes in different periods. The content of acid reached the maximum in S1 (June 3); The other seven components reached the maximum in S2 (July 3), and the results of principal component analysis also showed A. senticosus leaves collected in S2. The comprehensive scores of the eight active ingredients in leaves were the highest; The total accumulation of the eight active ingredients in different periods increased first and then decreased. During the period from S1 to S2, the total amount showed an upward trend, and reached the maximum at S2. Conclusion According to the changes of dry weight and eight active ingredients in A. senticosus leaves in different periods, the best harvest time is around S2 stage (from late June to early July) which provides basic information for determining the suitable harvest time of A. senticosus leaves.
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[基金项目]
国家“十三五”重点研发项目(2016YFC0500303);黑龙江省国家科技重大专项重点研发省级资助项目(GX17C006)