目的 HPLC法测定不同产地多茎重楼Paris polyphylla Smith var.yunnanensis根茎中重楼皂苷VⅡ、H、VI、Ⅱ、Ⅲ、I、V的含量，建立其HPLC指纹图谱，并与滇重楼根茎对照药材HPLC指纹图谱进行比较分析。方法 采用Thermore C₁₈色谱柱（250 mm×4.6 mm，5 μm）；流动相为水-乙腈，梯度洗脱，检测波长203 nm；柱温30℃；体积流量1 mL/min；进样量10μL，外标法与一测多评法同时对不同产地多茎重楼根茎中重楼皂苷VⅡ、H、VI、Ⅱ、Ⅲ、I、V的含量进行测定，采用单因素方差分析法对含量差异进行分析；建立多茎重楼根茎HPLC指纹图谱，并与滇重楼根茎对照药材HPLC指纹图谱进行比较分析。结果 不同产地多茎重楼根茎重楼皂苷VⅡ、H、VI、Ⅱ、Ⅲ、I、V的含量测定结果表明，在线性范围内线性关系良好（r>0.999 7），平均加样回收率为98.34%～99.34%，RSD≤1.00%；不同产地多茎重楼根茎中重楼皂苷VⅡ、H、VI、Ⅱ、Ⅲ、I、V的含量存在显著（P<0.05）或极显著（P<0.01）差异，重楼皂苷（I＋Ⅱ＋VI＋VⅡ）总量在1.239%～6.236%，显著高于《中国药典》规定的重楼药材质量控制标准的0.60%，且外标法与一测多评法测定结果无显著性差异。HPLC指纹图谱相似度评价结果表明，不同产地多茎重楼和滇重楼对照药材根茎中共有12个共有指纹特征峰，二者HPLC指纹图谱相似度较高。结论 该方法操作简单、准确、重复性好，可用于多茎重楼根茎中重楼皂苷VⅡ、H、VI、Ⅱ、Ⅲ、I、V的含量测定；不同产地多茎重楼根茎中重楼皂苷（I＋Ⅱ＋VI＋VⅡ）总含量较高，不同产地多茎重楼与滇重楼对照药材根茎HPLC指纹图谱相似度较高。

Objective HPLC was used to determine the content of the polyphyllins VⅡ, H, VI, Ⅱ, Ⅲ, I, and V in the rhizomes of multiple stems Paris polyphylla var. yunnanenesis from different places of origin, establishing HPLC fingerprint and comparative analysis HPLC fingerprint between multiple stems P. polyphylla var. yunnanenesis and P. polyphylla var. yunnanensis. Methods HPLC was performed on the column of Thermore C₁₈ with mobile phase of acetonitrile-water gradient at the flow rate of 1 mL/min; The detection wave-length was 203 nm, column temperature was 30℃, and volume was 10 μL; Content of multiple stems P. polyphylla var. yunnanenesis. was measured by external standard and quantitative analysis of multi-components (QAMS), and one-way ANOVA was used to explore its content difference. The HPLC fingerprint of rhizome from multiple stems P. polyphylla var. yunnanenesis was established and compared with that of rhizome from P. polyphylla var. yunnanensis. Results The determination results of 10 batches of rhizome of multiple stems P. polyphylla var. yunnanenesIs showed a good linear relationship in the linear range (r > 0.999 7), with an average recovery of 98.34%—99.34% and RSD ≦ 1.00%. There was a significant difference (P<0.05) or highly significant difference (P<0.01) about content of polyphyllins VⅡ, H, VI, Ⅱ, Ⅲ, I, and V among different places of origin in the rhizomes of multiple stems P. polyphylla var. yunnanenesis, the total content of polyphyllins (I+ Ⅱ + VI + VⅡ)% ranging from 1.239%—6.236%, which was significantly higher than 0.60% of the quality control standard of Paridis Rhizoma prescribed by Chinese Pharmacopoeia. No significant difference was observed between the results of external standard and quantitative analysis of multi-components methods. The HPLC fingerprint similarity evaluation results showed that there were 12 common fingerprint peaks between multiple stems P. polyphylla var. yunnanenesis and P. polyphylla var. yunnanensis, and with the high similarity of HPLC fingerprints. Conclusion The method was simple, accurate and reproducible, and can be used for the determination of polyphyllins VⅡ, H, VI, Ⅱ, Ⅲ, I, and V in multiple stems of P. polyphylla var. yunnanenesis, total content of polyphyllins (I + Ⅱ + VI + VⅡ)% in multiple stems of P. polyphylla var. yunnanenesis was relatively higher and HPLC fingerprints of rhizome of multiple stems P. polyphylla var. yunnanenesis and P. polyphylla var. yunnanensis from different places of origin also have high similarity.

国家自然科学基金资助项目（81360616）；云南省教育厅基金重点资助项目（2013Z153）