目的 比较红芪与炙红芪补中益气作用及成分差异，初步揭示红芪蜜炙炮制机制。方法 建立大鼠脾气虚模型，通过红芪与炙红芪不同剂量水提液干预，以大鼠血清木糖（D-xylose）、胃泌素（GAS）、白细胞介素-2（IL-2）、肿瘤坏死因子-α（TNF-α）为指标，对红芪与炙红芪补中益气药效作用进行比较。基于HPLC法分析红芪和炙红芪醇提液中的差异成分。结果 与对照组相比，模型组大鼠血清D-xylose、GAS水平均显著降低（P<0.01），与模型组相比，除红芪低剂量组外，各给药组大鼠血清D-xylose、GAS水平均显著升高（P<0.01），且炙红芪中剂量组D-xylose、GAS水平均显著高于红芪中剂量组（P<0.05、0.01）。与对照组相比，模型组大鼠血清IL-2和TNF-α水平均显著升高（P<0.01），与模型组相比，各给药组大鼠血清IL-2和TNF-α水平均显著降低（P<0.05、0.01）；与红芪中剂量组相比，炙红芪中剂量组大鼠血清IL-2和TNF-α水平均显著降低（P<0.05、0.01）。HPLC检测结果表明红芪炮制前后成分发生变化。结论 炙红芪和红芪均可以明显干预和改善大鼠脾气虚证，且炙红芪的药效作用优于红芪，炙红芪与红芪中存在差异成分。

Objective To compare the effects of Hedysari Radix and Hedysari Radix Praeparata Cum Melle in Buzhong Yiqi and its chemical composition, and preliminarily reveal the mechanism of Hedysari Radix processed with honey. Methods A rat model of spleen qideficiency was established. The rats were treated with different doses of water extracts of Hedysari Radix and Hedysari Radix Praeparata Cum Melle and the content of serum D-xylose, GAS, IL-2, TNF-α were used as indicators to study the differences in the efficacy of Hedysari Radix and Hedysari Radix Praeparata Cum Melle in Buzhong Yiqi. Based on HPLC techniques, the different components in methanol extract of Hedysari Radix and Hedysari Radix Praeparata Cum Melle were analyzed. Results Compared with the blank group, the serum xylose, gastrin content in the model group were significantly decreased. Compared with the model group, except for the low dose group of Hedysari Radix, the serum xylose, and gastrin content of the rats in each administration group were significantly increased (P<0.01), and the results in the middle dose group of Hedysari Radix were significantly higher than those in the middle dose group of Hedysari Radix Praeparata Cum Melle (P<0.05, P<0.01). Compared with the blank group, the serum IL-2 and TNF-α levels in the model group were significantly increased (P<0.01). Compared with the model group, the serum IL-2 and TNF-α levels in the rats in each drug group were significantly decreased (P<0.05, P<0.01); Compared with the medium dose group of Hedysari Radix, the serum IL-2 and TNF-α levels in the medium dose group of honey-processing Hedysari Radix were significantly decreased (P<0.05, P<0.01). There were differences in the chromatographic peaks in the fingerprints of Hedysari Radix and Hedysari Radix Praeparata Cum Melle. Conclusion Both Hedysari Radix and Hedysari Radix Praeparata Cum Melle can significantly intervene and improve the syndrome of spleen qi deficiency in rats and the pharmacodynamics effect of Hedysari Radix Praeparata Cum Melle is better than that of Hedysari Radix. There are differences in the components of Hedysari Radix and Hedysari Radix Praeparata Cum Melle and these differences may be the active substances that cause differences in the efficacy.

国家自然科学基金资助项目（81460611）；自然科学基金创新基地和人才计划项目（18JR3RA197）；甘肃省中药质量与标准研究重点实验室开放基金（ZYZL18-008）