[关键词]
[摘要]
目的 整体评价菊茎叶水提取物(JST)和乙醇提取物(JCT)对肠功能失调的改善作用及其机制。方法 应用脂多糖(LPS)制备兔肠功能失调模型,给予JST和JCT,应用1H-NMR技术研究兔粪便内源性代谢物变化规律。结果 相比对照组,模型组乳酸盐、甲酸盐显著增加,丙酸盐、谷氨酰胺、谷氨酸盐、天冬氨酸等显著下降;JST和JCT均能够增加天冬氨酸、腺嘌呤、苯丙氨酸、酪氨酸水平,同时降低甲酸盐水平。结论 代谢通路分析表明JST和JCT通过调节LPS所致紊乱的苯丙氨酸、酪氨酸和色氨酸生物合成,丙氨酸、天冬氨酸和谷氨酸盐代谢,以及甘氨酸、丝氨酸和苏氨酸代谢,发挥改善肠道功能紊乱的作用。
[Key word]
[Abstract]
Objective To holistically evaluate the protective effects of water and ethanol extracts of stems and leaves of Chrysanthemum morifolium and its underlying mechanism. Methods Gut dysfunction model was established by injection of liposaccharide in rabbits, and administrated with water and ethanol extracts of stems and leaves of C. morifolium to investigate the treatment. Feces of rabbits in each group were collected and analyzed by 1H-NMR complemented with multivariate statistical methods to evaluate the metabolic alteration. Results Compared with the control group, the levels of lactate and formate in liposaccharide intoxicated model group were significantly increased, and the concentrations of propionate, glutamine, glutamate, aspartate were notably decreased. Both the water and ethanol extracts of stems and leaves of C. morifolium ameliorated gut dysfunction of rabbits in a similar manner, increased the decreased levels of aspartate, adenine, phenylalanine, tyrosine induced by liposaccharide, and reduced the elevated content of formate. Conclusion Pathway analysis revealed that both the water and ethanol extracts of stems and leaves of C. morifolium could regulate the disturbed phenylalanine, tyrosine and tryptophan biosynthesis; disordered alanine, aspartate and glutamate metabolism and imbalanced glycine, serine and threonine amino acid metabolism, exerting a holistic protective effect on gut disorder. Thus, this study lays a scientific foundation for the resource utilization of stems and leaves of C. morifolium after the harvest of the inflorescence.
[中图分类号]
[基金项目]
国家自然科学基金青年基金项目(81603273);江苏省中药资源产业化过程协同创新中心资助项目(ZDXM-3-17);现代农业产业技术体系建设专项资金资助(CARS-21)