[关键词]
[摘要]
目的 研究地榆Sanguisorba officinalis水溶性化学成分。方法 采用大孔树脂(D101、HP-20)、透析袋、Toyopearl HW-40、反相硅胶RP18等柱色谱方法进行分离纯化,并运用1H-NMR、13C-NMR、ESI-MS等多种波谱方法对化合物进行结构鉴定。结果 从地榆10%乙醇部位中分离并鉴定了25个化合物,分别为4-(4'-hydroxyphenyl)-2-butanone-4'-O-β-D-glucopyranoside(1)、phenethanol-β-vicianoside(2)、junipetrioloside A(3)、citroside A(4)、corchoionoside C(5)、adenosine(6)、tryptophan(7)、tachinoside(8)、d-mandelic acid-β-D-glucopyranoside(9)、(+)-(7S,8S)-guaiacylglycerol 8-O-β-D-glucopyranoside(10)、biophenol(11)、3,5-dihydroxyphenethyl alcohol 3-O-β-glucopyranoside(12)、syringin(13)、(2E,5E)-3,7-dimethyl-2,5-octadiene-1,7-diol(14)、(±)-3-hydroxy-3,7-dimethyloct-6-enoic acid(15)、(2Z)-2,6-dimethyl-2,7-octadiene-1,6-diol(16)、phlorizin(17)、(+)-cyclo-olivil-6-O-β-D-glucopyranoside(18)、5'-methoxy-8'-hydroxyl-(+)-isolariciresinol-4'-O-β-D-glucopyranoside(19)、phenethyl-6-O-α-L-arabinofuranosyl-β-D-glucoside(20)、gaultherin(21)、benzyl-O-α-L-rhamnopyranosyl-(1→6)-β-D-glucopyranoside(22)、(2E)-7-hydroxy-3,7-dimethyl-2-octenyl-6-O-α-L-arabinofuranosyl-β-D-glucopyranoside(23)、3,3',4'-tri-O-methylellagic acid(24)、methyl-4-(β-D-glucopyranosyloxy)-3-hydroxy-5-methoxybenzoate(25)。结论 化合物1~22为首次从该植物中分离得到。
[Key word]
[Abstract]
Objective To study the chemical constituents in extract of Sanguisorba officinalis. Methods Compounds were isolated from 10% ethanol extracts by Macroporous resin (D101, HP-20), dialysis bag, reversed phase silica gel (RP-8, RP-18), Toyopearl HW-40 column chromatography and their structures were elucidated by NMR and MS data. Results Twenty-five compounds were isolated and their structures were elucidated as 4-(4'-hydroxyphenyl)-2-butanone-4'-O-β-D-glucopyranoside (1), phenethanol-β- vicianoside (2), junipetrioloside A (3), citroside A (4), corchoionoside C (5), adenosine (6), tryptophan (7), tachinoside (8), d-mandelic acid-β-D-glucopyranoside (9), (+) (7S,8S)-guaiacylglycerol 8-O-β-D-glucopyranoside (10), biophenol (11), 3,5-dihydroxyphenethyl alcohol 3-O-β-glucopyranoside (12), syringin (13), (2E,5E)-3,7-dimethyl-2,5-octadiene-1,7-diol (14), (±)-3-hydroxy-3,7-dimethyloct- 6-enoic acid (15), (2Z)-2,6-dimethyl-2,7-octadiene-1,6-diol (16), phlorizin (17), (+)-cyclo-olivil 6-O-β-D-glucopyranoside (18), 5'-methoxy-8'-hydroxyl-(+)-isolariciresinol-4'-O-β-D-gluco-pyranoside (19), phenethyl 6-O-α-L-arabinofuranosyl-β-D-glucoside (20), gaultherin (21), benzyl-O-α-L-rhamnopyranosyl-(1→6)-β-D-glucopyranoside (22), (2E)-7-hydroxy-3,7-dimethyl-2-octenyl 6-O-α-L-arabinofuranosyl-β-D-glucopyranoside (23), 3,3',4'-tri-O-methylellagic acid (24), and methyl-4-(β-D-glucopyranosyloxy)-3-hydroxy-5-methoxybenzoate (25). Conclusion Compounds 1-22 were isolated from this plant for the first time.
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[基金项目]
国家科技部新药创制重大专项:中药大品种技术改造与技术提升研究(地榆生白片技术改造)(2014ZX09201021-008);中药药效物质基础研究与创新中药发现四川省青年科技创新研究团队(2016TD0006);四川省科技厅支撑计划项目:中药种质资源库建设关键技术研究及示范(2014SZ0156)