[关键词]
[摘要]
目的 研究和比较全国7大产区不同产地酸枣仁药材中2种黄酮和2种皂苷的含量,筛选优质药材来源。方法 2016年和2017年2个年度对7大产区进行调查和样品收集,共收集样品74份,使用UPLC-UV-ELSD检测斯皮诺素和6'''-阿魏酰斯皮诺素2种黄酮,以及酸枣仁皂苷A和酸枣仁皂苷B 2种皂苷的含量。结果 2016、2017年采集样品中酸枣仁样品中斯皮诺素的质量分数分别为0.052%~0.102%和0.049%~0.144%,6'''-阿魏酰斯皮诺素质量分数分别为0.021%~0.072%和0.026%~0.088%,酸枣仁皂苷A的质量分数分别为0.016%~0.061%和0.033%~0.054%,酸枣仁皂苷B的质量分数分别为0.008%~0.046%和0.005%~0.046%。结论 经相关性分析,7个大产区间各成分含量基本上没有显著差异,斯皮诺素含量较高的产地有山西临汾(包括大宁和吉县)、河北涉县、河北迁安、甘肃庆阳、陕西眉县、陕西合阳、辽宁建昌、天津蓟县,酸枣仁皂苷A含量高的产地为山东各地,以及河北内丘和陕西渭南。综合比较山东各地2类成分含量普遍都高且变化较小。相关性分析也显示2种黄酮含量具有较好的相关性,而2种皂苷含量的相关性较差。
[Key word]
[Abstract]
Objective To study and compare the content of two flavonoids and two saponins in Ziziphi Spinosae Semen from seven major producing areas in China, and to provide high quality sources of medicinal materials. Methods Investigation and sample collection of seven major production areas were carried out in 2016 and 2017 and a total of 74 samples were collected. UPLC-UV-ELSD liquid phase method was used to determine the content of two flavones, spironol and 6'''-ferulinyl spironol, as well as two saponins jujuboside A and jujuboside B. Results In 2016 and 2017, the content of spinolin in the samples was 0.052%-0.102% and 0.049%-0.144%, respectively. The content of 6'''-ferulinyl spironol was 0.021%-0.072% and 0.026%-0.088%, respectively. The content of jujuboside A was 0.016%-0.061% and 0.033%-0.054%, respectively. The content of jujuboside B was 0.008%-0.046% and 0.005%-0.046%, respectively. Conclusion By correlation analysis, there was no significant difference in the content of each component among the seven major production areas. The producing areas with high spinosin content are Linfen, Shanxi (including Daning and Jixian), Shexian, Hebei, Qian' an, Hebei, Qingyang, Gansu, Meixian, Shaanxi, Heyang, Shaanxi, Jianchang, Liaoning, and Jixian, Tianjin. The producing areas with high content of jujuboside A are most places of Shandong province, Neiqiu, Hebei and Weinan, Shaanxi. Considering the content of two kinds of components, Shandong Province is generally high and has little change. The correlation analysis also showed that the content of two flavonoids had good correlation, but the content of two saponins had poor correlation.
[中图分类号]
[基金项目]
国家中药标准化项目:柴胡等9种中药饮片标准化建设——酸枣的野生抚育规范和标准研究(ZYBZH-Y-JIN-34)