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[摘要]
目的 分离鉴定海洋来源真菌Penicillium sp. H1的活性天然产物。方法 利用柱色谱等方法对次级代谢产物进行分离、纯化,运用质谱、核磁共振和比旋光等手段鉴定化合物的结构,采用药敏纸片法评价化合物的抗菌活性。结果 从海洋真菌Penicillium sp. H1的发酵产物中分离得到了5个化合物,分别鉴定为炭角菌素E(1)、烟曲霉素C(2)、震颤真菌毒素(3)、penicillide(4)以及啶南平A(5)。化合物1对香蕉枯萎病菌Fusarium oxysporum f. sp. cubense有中等的抑制活性,最小抑制浓度(MIC)值为32.0 μg/mL;化合物2对金黄色葡萄球菌Staphylococcus aureus有抑制活性,MIC值为64.0μg/mL。结论 化合物1为新化合物,化合物1和2具有一定的抗菌活性。
[Key word]
[Abstract]
Objective To isolate and identify the active natural products of marine-derived Penicillium sp. H1. Methods The isolations and purifications of secondary metabolites were performed by means of column chromatography over silica gel. And their structures were elucidated through the spectroscopic analysis of MS, NMR, and specific rotations. The bioactivities were assayed by paper diffusion. Results From the fermentation broth of marine-derived Penicillium sp. H1, five compounds were isolated and identified as xylarinonericin E (1), fumitremorgin C (2), verruculogen (3), penicillide (4), and pyripyropene A (5), respectively. Compound 1 showed moderate antifungal activity against Fusarium oxysporum f. sp. cubense with MIC value of 32.0 μmol/mL. Compound 2 displayed antibacterial activity against Staphylococcus aureus with MIC value of 64.0 μg/mL. Conclusion Compound 1 is a new compound. Compounds 1 and 2 exhibit moderate antimicrobial activities.
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[基金项目]
广西民族大学引进人才科研启动项目(2017KJQD004);广西民族大学青年基金项目(2018MDQN003);2018年广西高校中青年教师基础能力提升项目(2018KY0177);广西科技基地和人才专项(桂科AD18126005);广西创新驱动发展重大专项子课题(桂科AA17204087-21);国家自然科学基金项目(21662006);中国博士后科学基金面上项目(2016M602920XB);广西科技部重点资助项目(AB16890009)