目的 研究不同治法中药配伍调节肾上腺皮质功能的作用。方法 小鼠ig给予1.65 mg/（kg·d）氢化可的松14 d诱发药源性证候，同步给予助阳散寒（附子-肉桂）、滋阴泻火（知母-黄柏）、补气生津（人参-黄芪）、补血益精（熟地-山茱萸）4种中药药对干预，检测小鼠体质量、抓力、腋温、旷场活动度、躯体不同部位红外温度等表征信息；取脾脏、胸腺称定质量并计算脏器指数；运用ELISA法检测血清皮质酮、促肾上腺皮质激素（ACTH）、肾上腺素和去甲肾上腺素含量；采用实时荧光定量PCR技术检测肾上腺类固醇合成急性调节蛋白（StAR）、胆固醇侧链裂解酶（Cyp11a1）、21-羟化酶（Cyp21a1）、11β-羟化酶1（Cyp11b1）和11β-羟化酶2（Cyp11b2）、酪氨酸羟化酶（Th）、多巴胺脱羧酶（Ddc）、多巴胺β-羟化酶（Dbh）、苯乙胺-N-甲基转移酶（Pnmt）基因表达；运用Western blotting方法检测肾上腺低密度脂蛋白受体（LDLR）、B族I型清道夫受体（SR-BI）、StAR蛋白表达。结果 4种治法对氢化可的松造成的小鼠体质量下降和体温下降效果不显著，附子-肉桂组小鼠抓力显著高于模型组（P<0.05）。与模型组比较，知母-黄柏组小鼠血清皮质酮含量显著升高（P<0.01），StAR、Cyp11b1、Cyp11b2基因表达水平显著升高（P<0.05、0.01），SR-BI、StAR蛋白表达水平均显著升高（P<0.01）。结论 滋阴泻火治法寒性药对（知母-黄柏）对小剂量氢化可的松诱发的药源性证候小鼠肾上腺皮质功能的纠正作用最佳。

Objective To study the effects of different Chinese materia medica combinations on the regulation of adrenal function. Methods Mice with 1.65 mg/(kg·d) hydrocortisone for 14 d to induce drug-induced syndrome was treated synchronously with assissting yang and dissipating cold (Aconm Lateralis Radix Praeparaia-Cinnamomi Cortex), nourishing yin and reducing fire (Anemarrhenae Rhizoma-Phellodendri Chinensis Cortex), tonifying qi and promoting fluid production (Ginseng Radix et Rhizoma-Astragali Radix), and tonifying blood and benefiting spirit (Rehmanniae Radix Praeparata-Corni Fructus). The body mass of mice was dynamically observed every day, and the characteristic information such as body mass, holding power, axillary temperature, activity degrees of open field, and infrared temperature were detected by the experimental methodology of mice syndrome differentiation; The mice were sacrificed whose spleen, thymus was weighed and the organ index was calculated. Serum corticosterone, ACTH, adrenaline, and noradrenaline were measured by ELISA. The gene expressions of Star, Cyp11a1, Cyp21a1, Cyp11b1, Cyp11b2, Dbh, Ddc, Pnmt, and Th in the adrenal were detected by real-time fluorescent quantitative PCR. The protein's expressions of LDLR, SRB1, and StAR were detected by Western blotting. Results The effects of four different treatments on the reduction of body weight and body temperature caused by hydrocortisone were not significant. The holding power of mice in Aconm Lateralis Radix Praeparaia-Cinnamomi Cortex group was significantly higher than that of hydrocortisone group (P < 0.05). Compared with the hydrocortisone group, the serum corticosterone content of mice in Anemarrhenae Rhizoma-Phellodendri Chinensis Cortex group increased significantly (P < 0.01); The genes expression of Star, Cyp11b1, and Cyp11b2 was significantly up-regulated (P < 0.05, 0.01); The proteins expression of SRBI and StAR were significantly up-regulated. Conclusion The nourishing yin and purging fire treatment group (Anemarrhenae Rhizoma-Phellodendri Chinensis Cortex) is the best to correct and protect the adrenal cortex function in mice with hydrocortisone induced syndrome at low dose.

R285.5

国家自然科学基金面上项目（81473562）；国家自然科学基金面上项目（81873212）