目的 探讨连翘苷抑制人肾细胞腺癌786-0细胞生长、迁移及侵袭的作用机制。方法 体外培养786-0细胞，在其中加入不同浓度连翘苷进行干预。MTT法检测细胞生存活力，AO/EB法检测细胞凋亡，划痕实验与Transwell实验分别考察细胞迁移、侵袭能力。Western blotting法检测细胞内PI3K、p-PI3K、Akt、p-Akt、FOXO3a、p-FOXO3a、p21、p27、Fasl、Bim、MMP-2及MMP-9蛋白表达情况。结果 连翘苷可有效抑制肾癌细胞生长、促进凋亡，干扰细胞周期，上调p21、p27、Fasl及Bim表达水平，与对照组比较差异明显（P<0.05、0.01）；与对照组比较，不同浓度连翘苷可明显抑制肾癌细胞迁移与侵袭，减少MMP-2、MMP-9合成（P<0.05、0.01）；同时连翘苷能明显抑制PI3K、Akt、FOXO3a磷酸化（P<0.05、0.01），且呈浓度依赖性。结论 连翘苷可通过PI3K/Akt信号通路调控786-0细胞凋亡与细胞周期、抑制肾癌细胞生长；同时连翘苷经PI3K/Akt通路可有效削弱肾癌细胞迁移与侵袭能力。

Objective To investigate the mechanism of forsythin in inhibiting the growth, migration and invasion of human renal cancer cells (786-0). Methods Human renal cancer cells (786-0) were cultured in vitro, and different concentrations of forsythin were added. Cell viability was detected by MTT assay, cell apoptosis was detected by AO/EB assay, cell migration and invasion abilities were respectively investigated by wound healing and transwell migration assays. The expression levels of PI3K, p-PI3K, Akt, p-Akt, FOXO3a, p-FOXO3a, p21, p27, Fasl, Bim, MMP-2, and MMP-9 were detected by Western bloting. Results In 786-0 cells, forsythin effectively inhibited the growth of renal cancer cells, promoted apoptosis, interfered with cell cycle, and upregulated expression levels of p21, p27, Fasl, and Bim, when compared with control group (P < 0.05, 0.01); Compared with the control group, different concentrations of forsythin can significantly inhibit the migration and invasion of renal cancer cells, and reduce the synthesis of MMP-2 and MMP-9 (P < 0.05, 0.01). Meanwhile, forsythin can significantly inhibit the phosphorylation of PI3K, Akt and FOXO3a in a concentration-dependent manner (P < 0.05, 0.01). Conclusion Forsythin can regulate apoptosis and cell cycle, and inhibit the growth of renal cancer cells by down-regulating the PI3K/Akt signaling pathway. At the same time, forsythin can effectively inhibit the ability of renal cell migration and invasion through the PI3K/Akt pathway.

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