[关键词]
[摘要]
目的 研究三七Panax notoginseng中的微量皂苷成分,为三七化学成分的系统研究、质量控制及安全性评价提供基础。方法 综合利用MCI和ODS柱色谱以及制备型高效液相色谱等技术进行分离纯化,通过谱学数据分析以及与文献比对进行化合物结构鉴定。结果 从三七根茎中共分离得到12个单体化合物,分别鉴定为三七皂苷P1(1)、三七皂苷T5(2)、人参皂苷Rk3(3)、人参皂苷Rh4(4)、三七皂苷T3(5)、20(S)-原人参三醇(6)、达玛-20(21),24-二烯-3β,6α,12β-三醇(7)、人参皂苷Rg3(8)、七叶胆皂苷XⅢ(9)、人参皂苷Rk1(10)、人参皂苷Rg5(11)、20(S)-人参皂苷Rh2(12)。结论 化合物1为1个新的达玛烷型三萜皂苷。
[Key word]
[Abstract]
Objective To study the minor triterpenoid saponins from the roots of Panax notoginseng, which provided basis for the systematic research, quality control and safety evaluation of P. notoginseng. Methods The compounds were isolated and purified by MCI resin, ODS, along with Preparative-HPLC, and the structures were identified by spectroscopic analysis, and comparing with the pubished literature values. Results Twelve monomeric compounds isolated from the roots of P. notoginseng, were identified as notoginsenoside P1 (1), notoginsenoside T5 (2), ginsenoside Rk3 (3), ginsenoside Rh4 (4), notoginsenoside T3 (5), 20(S)-protopanaxatriol (6), dammar 20 (21),24-diene-3β,6α,12β-triol (7), ginsenoside Rg3 (8), gypenoside XⅢ (9), ginsenoside Rk1 (10), ginsenoside Rg5 (11), and 20 (S)-ginsenoside Rh2 (12). Conclusion Compound 1 is a new dammarane-type triterpenoid saponin.
[中图分类号]
R284.1
[基金项目]
国家自然科学基金青年项目(81703934);黑龙江省教育厅项目资助(1353MSYYB010);牡丹江师范学院国家级课题培育项目(GY201307,GP201609)