[关键词]
[摘要]
目的 挖掘夏枯草中可能调控三萜和酚酸类产物生物合成的MYB转录因子。方法 从夏枯草转录组数据库中筛选MYB转录因子,并对蛋白基序、理化性质、功能注释、系统进化、表达特性等进行分析,并预测其功能。结果 在夏枯草转录组水平上共鉴定出参与27个MYB转录因子基因,c32045.graph_c0可能通过对肉桂酸4-羟化酶基因表达的抑制作用,阻碍迷迭香酸的生物合成,从而作为转录抑制子对酚酸类产物生物合成起负调控作用。c26895.graph_c0可能通过调节三萜类和迷迭香酸类产物合成过程中的代谢中间产物的流向,从而阻碍三萜类和酚酸类的生物合成。结论 该研究预测了调控夏枯草三萜类和酚酸类产物生物合成的相关MYB转录因子,也为进一步研究夏枯草MYB转录因子在次生代谢产物中的调控功能奠定了基础。
[Key word]
[Abstract]
Objective To identify the MYB transcription factors that may regulate the biosynthesis of triterpenoids and phenolic acids in Prunella vulgaris. Methods MYB transcription factor were identified from the P. vulgaris transcriptome database, their motif, physical and chemical properties, functional annotation, family evolution and expression patterns were examined. Results A total of 27 MYB transcription factors were identified. c32045.graph_c0 might inhibit the biosynthesis of rosmarinic acid by inhibiting the expression of cinnamic acid 4-hydroxylase gene, thus acting as a transcription suppressor to negatively regulate the biosynthesis of phenolic acids. c26895.graph_c0 might inhibit the biosynthesis of triterpenoids and phenolic acids by regulating the flow of metabolic intermediates in the biosynthesis of triterpenoids and rosmarinic acids. Conclusion The candidate MYB transcription factors related to the biosynthesis of triterpenoids and phenolic acids were obtained. It also laid a foundation for the further study of MYB in regulating of secondary metabolites in P. vulgaris.
[中图分类号]
R282.12
[基金项目]
国家自然科学基金资助项目(81603232);国家重点研发计划(2017YFC1702800);河南省重大科技专项(171100310500);河南中医学院博士科研基金(BSJJ2015-13);河南省科技攻关项目(172102310539)