[关键词]
[摘要]
目的 建立香鳞毛蕨Dryopteris Fragrans有效部位中10种间苯三酚类成分的一测多评测定方法。方法 采用高效液相色谱法,以绵马素BB为内参物,测定其与绵马素PB、绵马素AB、黄绵马酸BB、田基黄绵马酸A、黄绵马酸PB、异黄绵马酸PB、黄绵马酸AB、Compound VI和绵马酚B的相对校正因子,采用相对校正因子计算这9种间苯三酚成分的量,实现一测多评。同时采用外标法测定有效部位中该10种成分的量,并比较2种测定方法的差异,以验证一测多评法的可行性和准确性。结果 各相对校正因子重复性良好,12批有效部位中10种间苯三酚成分量的计算值与实测值无显著性差异。结论 在缺少对照品的情况下,以绵马素BB为内参物,建立的一测多评法可用于香鳞毛蕨有效部位的定量分析,为香鳞毛蕨多指标成分质量评价提供参考。
[Key word]
[Abstract]
Objective To establish quantitative analysis of multi-components with single marker (QAMS) for determination of 10 phloroglucinol contents in effective fraction of Dryopteris fragrans. Methods The relative correction factors of nine phloroglucinol (aspidin PB, aspidin AB, flavaspidic acid BB, saroaspidin A, flavaspidic acid PB, disflavapidic acid PB, flavaspidic acid AB, compound VI, and aspidinol B) were determined by HPLC method with the aspidinn BB as the internal standard, which were to calculate the content of each. At the same time, external standard method (ESM) was used to determine the contents of 10 components in effective fraction, and the differences between the two methods were compared to verify the feasibility and accuracy of QAMS method. Results The relative correction factor (RCF) was good. There was no significant difference between the quantitative results with the two methods in the 12 batches of 10 phloroglucinols. Conclusion The established QAMS method can be used for quantitative analysis of D. fragrans with aspidinn BB as the internal standard in the absence of reference substances, and provided a reference for the multi-index quality evaluation in effective fraction of D. fragrans.
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[基金项目]
广东省应用型科技研发专项(2015B020234009);国家中医药行业科技专项(201507004);广东药科大学“创新强校工程”资助项目(2016KZDXM039)