目的 制备具有缓释作用的姜黄素固体脂质纳米粒（curcumin solid lipid nanoparticles，Cur-SLN）和长循环固体脂质纳米粒（long-circulating solid lipid nanoparticles，LSLN），并对2种纳米粒的理化性质进行考察。方法 采用乳化-超声法制备Cur-SLN，并对最优处方下制备的Cur-SLN进行包封率和载药量的测定，采用后插法制备Cur-LSLN，并考察Cur-SLN和Cur-LSLN的粒径、Zeta电位，差示扫描量热法（DSC）分析姜黄素在纳米粒中的存在状态，透射电镜观察两者的形态，透析法进行体外释放实验。结果 最优处方下制备的Cur-SLN和Cur-LSLN的外观为球形及类球形，包封率分别为（89.15±0.66）%、（92.97±0.27）%，载药量分别为（1.72±0.08）%、（1.98±0.08）%，粒径分别为（144.5±4.1）、（155.0±2.6）nm，Zeta电位分别为（-23.6±0.2）、（-47.8±1.8）mV，通过DSC检测，可确定纳米粒中的Cur已转变为无定形态，体外释放实验结果显示，2种制剂的药物释放分为突释期和缓释期，均在12 h内释放较快，Cur-SLN在96 h累积释放86.63%，Cur-LSLN在96 h累积释放76.98%，Cur-LSLN表现出更好的缓释效果。结论 采用乳化-超声法可成功制备Cur-SLN和Cur-LSLN，PEG修饰后的纳米粒有更好的缓释性能，可延长药物在体内存在的时间，为靶向药物的开发做了铺垫。

Objective The sustained release curcumin solid lipid nanoparticles (Cur-SLN) and long circulating solid lipid nanoparticles (LSLN) were prepared, and the physicochemical properties of the two nanoparticles were investigated. Methods Cur-SLN was prepared by emulsification ultrasonic method, and then the entrapment efficiency and drug loading of Cur-SLN prepared under the optimal formulation were determined. Cur-LSLN was prepared by back-extrapolation method, and the physicochemical properties of Cur-SLN and Cur-LSLN were evaluated by entrapment efficiency, drug loading, particle size, and Zeta potential; DSC was used to analyze, in vitro release characteristics and the transmission electron microscope (TEM) was used to observe particle appearance. Results Based on the optimal conditions, TEM showed that the appearance of Cur-SLN and Cur-LSLN were spherical or nearly spherical, the entrapment efficiency respectively were (89.15 ±0.66)% and (92.97 ±0.27)%, drug loading were (1.72 ±0.08)% and (1.98 ±0.08)%, average diameters of particles were (144.5 ±4.1) nm and (155.0 ±2.6) nm, and the mean Zeta potential were (−23.6 ±0.2) mV and (−47.8 ±1.8) mV. Through DSC detection, it can be determined that Cur in nanoparticles had been transformed into amorphous state. In vitro release test showed that the drug release of the two preparations was divided into two stages: burst release phase and sustained released stage, the release rate was fast in 12 h, and the cumulative release of Cur-SLN in 96 h was 86.63%, and Cur-LSLN was 76.98%, so Cur-LSLN showed better sustained-release effect. Conclusion Cur-SLN and Cur-LSLN can be successfully prepared by emulsification ultrasonic method, and PEG modified nanoparticles have better sustained-release properties and prolong the time of the presence of drug in vivo, providing reference for the development of targeted drugs.

国家自然科学基金资助项目（81202192）；河北省中医药管理局项目（2013075）；河北省卫生厅项目（20130034）