[关键词]
[摘要]
目的 通过响应面法研究和优化刺五加根废弃物多糖的提取工艺。方法 采用苯酚-硫酸法对刺五加根废弃物多糖进行定量分析,在单因素实验的基础上,运用响应面法设计对刺五加根废弃物多糖的提取工艺进行优化。结果 响应面优化所得最好条件分别为温度80℃、提取时间1.5 h、液料比21,在最优条件下多糖的平均提取率为10.14%。结论 响应面法对刺五加根废弃物多糖的提取条件优化合理,为后续刺五加根废弃物的再资源化开发利用提供一定的理论基础。
[Key word]
[Abstract]
Objective To study and optimize the extraction technology of polysaccharides from Acanthopanax senticosus (AS) waste residue by response surface methodology (RSM). Methods Phenol-sulfuric acid method was used to quantitatively analyze polysaccharides from AS waste residue. Based on single factor, the extraction process factors of polysaccharides from AS waste residue were optimized by RSM. Results The best conditions for the response surface optimization were as follows:extraction temperature was 80℃, extracting time was 1.5 h, and liquid-material ratio was 21. The extraction rate of polysaccharide was 10.14% under the optimal conditions. Conclusion The extraction conditions of the polysaccharide from AS are optimized by response surface method. This data can provide a theoretical basis for the further exploitation and utilization of the subsequent waste residue of AS.
[中图分类号]
R284.2
[基金项目]
中国博士后科学基金(2015M581467);黑龙江省博士后特别资助(LBH-TZ1613);黑龙江省博士后科学基金(LBH-Z15107);哈尔滨市青年科技创新人才专项基金(2013RFQXJ150);黑龙江省青年创新人才培养计划(UNPYSCT-2015017);哈尔滨商业大学校级科研项目(17XN061);哈尔滨商业大学研究生创新科研基金项目(YJSCX2018-544HSD)