目的 研究犀角地黄汤加味对败血症导致的炎症和肺损伤的影响，探讨其对核转录因子-κB（NF-κB）信号通路的调控作用。方法 制备败血症小鼠模型，测量血清中乳酸脱氢酶（LDH）反映败血症小鼠的器官损伤程度；HE染色观察败血症小鼠肺组织病理学变化；酶联免疫吸附法测定肺组织中细胞因子白细胞介素-6（IL-6）、白细胞介素-1β（IL-1β）、小鼠巨噬细胞炎症蛋白-2（MIP-2）水平；免疫组化法检测肺组织中粒细胞分化抗原-1（Gr-1）的阳性表达；TUNEL法分析败血症小鼠肺中的TUNEL阳性细胞数量；Western blotting法检测肺组织中NF-κB p65蛋白表达。结果 犀角地黄汤加味减轻了败血症小鼠的炎症反应和肺损伤程度，以及嗜中性粒细胞浸润。犀角地黄汤加味显著减少了败血症小鼠肺中的TUNEL阳性细胞数量。结论 犀角地黄汤加味可以减轻败血症导致的炎症和肺损伤，可能是通过抑制NF-κB信号通路发挥作用。

Objective To investigate the effect of Xijiao Dihuang Decoction on inflammation and lung injury caused by sepsis in children and explore its regulatory effect on NF-κB signaling pathway. Methods The mice model with pediatric sepsis was establishen. Lactate dehydrogenase was used to detect organ damage and inflammation in sepsis mice. HE staining was used to observe the pathological changes of lung tissue in sepsis mice. Interleukin-6 (IL-6), Interleukin-1β (IL-1β), and MIP-2 content were measured by enzyme-linked immunosorbent assay. The expression of Gr-1 in lung tissues was determinated by immunohistochemistry. the number of TUNEL-positive cells in the lungs of sepsis mice was analyzed by TUNEL method; Western blotting was used to detect the expression of NF-κB p65 in lung tissue. Results The addition of Xijiao Dihuang Decoction reduced inflammation and lung injury, as well as neutrophil infiltration in septicemic mice. In addition, Xijiao Dihuang Decoction significantly reduced TUNEL-positive cells. Conclusion Xijiao Dihuang Decoction can reduce inflammation and lung injury caused by sepsis in children by inhibiting NF-κB signaling pathway.

R285.5