目的 探讨贝母素乙对人肺癌A549细胞侵袭及迁移的影响。方法 采用终浓度为0、50、100、200 μmol/L贝母素乙处理A549细胞，通过细胞侵袭实验、细胞划痕实验、实时荧光定量PCR（qRT-PCR）法、ELISA法及Western blotting实验检测贝母素乙对A549细胞侵袭、迁移的影响及潜在机制。结果 与对照组比较，贝母素乙各浓度组的A549细胞穿膜数、细胞间划痕的愈合率及MMP-9、MMP-2表达显著下降（P<0.01）；与对照组比较，贝母素乙各浓度组的A549细胞E-cadherin mRNA表达明显增加（P<0.01），而N-cadherin和vimentin mRNA表达均显著降低（P<0.01）；与对照组比较，除50 μmol/L贝母素乙处理A549细胞24 h后的FN蛋白表达无显著性变化外（P>0.05），其余贝母素乙各浓度组在各时间点上的FN蛋白表达均显著下降（P<0.05、0.01）；与对照组比较，贝母素乙各浓度组的p-PI3K/PI3K、p-mTOR/mTOR值及100、200 μmol/L贝母素乙组的p-Akt/Akt值显著下降（P<0.05、0.01）。结论 贝母素乙具有抑制A549细胞侵袭及迁移能力的作用，该作用与贝母素乙调控PI3K/Akt/mTOR通路活性进而减缓上皮-间质转化（EMT）进程有关。

Objective To detect the influence of peiminine on the invasion and migration of human lung cancer A549 cells. Methods A549 cells were treated with peiminine at the final concentrations of 0, 50, 100, and 200 μmol/L, respectively. The influence of peiminine on the invasion and migration of A549 cells and its underlying mechanisms were investigated by cell invasion experiment, cell scratch experiment, real-time quantitative PCR, ELISA, and Western blotting. Results Compared with 0 μmol/L peiminine group, the cell transmembrane number and scratch wound healing rate and expressions of MMP-9 and MMP-2 in the group treated with different concentrations of peiminine significantly decreased (P < 0.01). Compared with 0 μmol/L peiminine group, the mRNA expression of E-cadherin increased significantly (P < 0.01), while the mRNA expressions of N-cadherin and vimentin decreased significantly (P < 0.01). Compared with 0 μmol/L peiminine group, FN protein expression was significantly decreased in all the groups with different concentrations of peiminine group except treatment with 50 μmol/L peiminine after 24 h (P < 0.05, 0.01). Compared with 0 μmol/L peiminine group, the ratio of p-PI3K/PI3K and p-mTOR/mTOR in all concentrations of peiminine groups and p-Akt/Akt in 100 and 200 peiminine groups were significantly decreased (P < 0.05, 0.01). Conclusion Peiminine can inhibit the invasion and migration of A549 cells, which may be related to the activation of PI3K/Akt/mTOR pathway and decreasing the epithelial-mesenchymal transition process.

R285.5