目的 研究确定当归原生粉乙醇灭菌的最优工艺条件并对其品质进行研究。方法 以灭菌率为考察指标，乙醇用量、乙醇体积分数、灭菌温度、灭菌时间为因素，在单因素试验的基础上，采用响应面法对灭菌工艺条件进行优化；以菌含量、挥发油提取率及阿魏酸含量为指标，评价优化的乙醇灭菌工艺，以及湿热灭菌法及干热灭菌法对当归原生粉灭菌前后品质的影响。结果 最优的乙醇灭菌工艺为乙醇用量34%、乙醇体积分数80%、灭菌温度78℃，灭菌率达99.59%；3种不同灭菌方式的灭菌率为乙醇灭菌法 > 湿热灭菌法 > 干热灭菌法；乙醇灭菌的当归原生粉挥发油提取率相比未处理组提高了94.44%，且所含的阿魏酸仅减少8%，而湿热灭菌及干热灭菌处理后挥发油提取率分别下降了55.56%及100%，且阿魏酸完全被破坏。结论 响应面法优化的乙醇灭菌工艺能保证当归原生粉微生物含量达要求，并且品质较常规灭菌法优，有望替代当归原生粉的常规灭菌技术。

Objective To optimize the conditions of the ethanol sterilization technology for Angelicae Sinensis Radix (ASR) primary powder and compare of its quality. Methods With the sterilization rate as the index and ethanol usage, ethanol concentration, sterilization temperature and sterilization time as factors, the response surface methodology based on the single-factor experiments was employed to optimize the ethanol sterilization process conditions. With the microbial content, extraction rate of volatile oil and the content of ferulic acid as the indexes, the effect of optimized ethanol sterilization process, the moist heat sterilization method and the dry heat sterilization method on the quality of the sterilization before and after of ASR primary powder were evaluated. Results The optimal parameters of ethanol sterilization technology were as follows:dosage of ethanol of 34%, ethanol concentration of 80% and sterilization temperature of 78℃, the sterilization rate was 99.59%. Comparing with the moist heat sterilization method and the dry heat sterilization method, ethanol sterilization method had some advantages, such as higher sterilization rate and better overall yield. Compared with the untreated product, the extraction rate of volatile oil increased 94.44% and the content of ferulic acid decreased 8% after the ethanol sterilization, while decreased 55.56% and 100% of extraction rate of volatile oil after the moist heat sterilization and the dry heat sterilization, respectively; And ferulic acid was destroyed completely. Conclusion The microbial indicators reached the requirement and the quality in chemical composition of ASR primary powder treated with the optimization of the ethanol sterilization process by the response surface method is better than that of conventional sterilization methods. It is expected to be an alternative to conventional sterilization methods of ASR primary powder.

R283.6

江西省教育厅科技项目（GJJ160859）；江西省卫计委科技项目（2016A044）；江西中医药大学重点学科青年教师培养计划项目（2016jzzdxk007）；江西民族传统药现代科技与产业发展协同创新中心基金（JXXT201403017）；江西省杰出青年人才项目（20162BCB23035）