目的 研究亳芍内生真菌Alternaria alternate的次生代谢产物。方法 利用硅胶、SephadexLH-20、反相、中压、高效液相制备等多种色谱法分离化合物，采用波谱方法对化合物进行结构鉴定。结果 从内生真菌Alternaria alternate次生代谢产物中分离得到15个化合物，分别鉴定为methyl 4-acetamido-3-hydroxybenzoate（1）、（E）-methyl-5-hydroxy-3-methylpent-2-eno（2）、异苯并呋喃酮A（3）、对羟基苯乙酮（4）、6-hydroxy-isosclerone（5）、talaroflavone（6）、7-hydroxy-2-hydroxymethyl-5-methyl-4H-chromen-4-one（7）、alternarienonic acid（8）、7-hydroxy-2，5-dimethy-4H-1-benzopyran-4-one（9）、5-hydroxy-epialtenuene（10）、交链孢醇（11）、methyl 3-hydroxybenzoate（12）、stemphyperylenol（13）、细格菌素（14）、交链孢烯（15）。结论 其中化合物1、3、5～10、12～13为首次从内生真菌Alternaria alternate次生代谢产物中分离得到。

Objective This study focused on the secondary metabolites of endophytic fungus Alternaria alternate in Paeonia lactiflora. Methods Compounds were isolated from the EtOAc extract by chromatography technology and their structures were elucidated on the basis of comprehensive spectroscopic analysis. Results A total of 15 compounds were isolated and their structures were identified as methyl 4-acetamido-3-hydroxybenzoate (1), (E)-methyl5-hydroxy-3-methylpent-2-eno (2), isobenzofuranone A (3), 4-hydroxyacetophenone (4), 6-hydroxy-isosclerone (5), talarojlavone (6), 7-hydroxy-2-hydroxymethyl-5-methyl-4H-chromen-4-one (7), alternarienonic acid (8), 7-hydroxy-2,5-dimethyl-4H-1-benzopyran-4-one (9), 5-hydroxy-epialtenuene (10), alternariol (11), methyl 3-hydroxybenzoate (12), stemphyperylenol (13), altenusin (14), and altenuene (15). Conclusion Compounds 1, 3, 5 -10, 12, and 13 are isolated from Alternaria alternatefor the first time.

安徽省科技重大专项（18030801128）；安徽省中央引导地方科技发展专项（2017070702D173）；安徽省高等学校自然科学基金2019年度重点项目（KJ2019A）；植物化学与西部植物资源持续利用国家重点实验室资助项目（P2017-KF03）