[关键词]
[摘要]
目的 建立同时测定不同产地牡丹皮中没食子酸、5-羟甲基糠醛、没食子酸甲酯、氧化芍药苷、儿茶素、芍药苷、1,2,3,6-O-四没食子酰葡萄糖、1,2,4,6-O-四没食子酰葡萄糖、苯甲酸、1,2,3,4,6-O-五没食子酰葡萄糖、牡丹皮苷C、苯甲酰氧化芍药苷、丹皮酚13种化学成分的HPLC方法。方法 采用50%甲醇回流提取,色谱条件采用C18柱(250 mm×4.6 mm,5 μm),以乙腈-0.5%磷酸水溶液为流动相,梯度洗脱,体积流量1.0 mL/min,检测波长254 nm,柱温30℃。结果 所测13种化学成分在测定的质量浓度范围内线性关系良好,r均大于0.999 5,有良好的精密度、稳定性、重复性和回收率。结论 所建立的HPLC方法可用于同时测定牡丹皮中13种化学成分的含量,该方法高效、准确、重复性好,可用于牡丹皮药材的质量控制。
[Key word]
[Abstract]
Objective To establish an HPLC method for the analysis of thirteen main chemical constituents (gallicacid, 5-hydroxymethylfurfural, methylgallate, oxypaeoniflora, catechins, paeoniflorin, 1,2,3,6-tetra-O-galloyl-D-glucose, 1,2,4,6-tetra-O-galloyl-D-glucose, benzoicacid, 1,2,3,4,6-penta-O-galloyl-D-glucose, mudanpioside C, benzoyloxypaeoniflorin, and paeonal) in Moutan Cortex from different origins. Methods The methanol (50%) reflux extraction was adopted to Moutan Cortex. Separation was carried out on C18 (250 mm×4.6 mm, 5 μm), the mobile phase was eluted with acetonitrile-0.5% phosphoric acid. The flow rate was 1.0 mL/min, the detection wavelength was 254 nm, and the column temperature was 30℃. Results The linear relation of thirteen main active components measured in the range of mass concentration was good with perfect precision, repeatability, and stability, the value of which was all more than 0.999 5. Conclusion The HPLC method established for simultaneous determination of thirteen main chemical compositions in Mortan Cortex is effective, accurate, and reproducible, which can be used for the quality control of Mortan Cortex.
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[基金项目]
安徽省科技重大专项(1603081113);安徽省教育厅自然科学重点项目(KJ2015A388);国家中医药管理局中药标准化项目(国中医药办科技函[2016]号);亳州市创新创业领军人才行动计划(亳组2016[32]号);安徽省农业科技创新资金项目(科农秘2017[344]号)