目的 克隆1条红花AP2/ERF家族转录因子编码区序列，并构建植物表达载体。方法 在红花转录组测序基础上，利用RT-PCR方法克隆1条红花AP2/ERF家族转录因子编码区序列（CtERF1）并进行生物信息学分析，利用ClustalW 1.83软件构建系统进化树，在CtERF1基因序列两端引入Spe I和XbaI酶切位点，构建含有35 S启动子的植物超表达载体pBASTA-CtERF1。结果 CtERF1基因编码297个氨基酸，且具有典型的AP2/ERF基因的功能结构域，含有1个AP2区域，为ERF亚族蛋白，亚细胞定位分析推测其定位在细胞质和细胞核上。系统进化分析表明，CtERF1基因编码氨基酸与其他物种氨基酸具有一定的同源性，其中与杨树和人参的亲缘关系最近。通过分子生物学方法，成功构建pBASTA-CtERF1植物表达载体。结论 成功克隆了1条红花AP2/ERF家族转录因子编码区基因CtERF1，并构建了植物表达载体pBASTA-CtERF1。

Objective To clone a coding region sequence of AP2/ERF transcription factor family from Carthamus tinctorius, and construct a plant expression vector. Methods A gene (CtERF1) of AP2/ERF family transcription factor was cloned by RT-PCR based on the sequence of C. tinctorius transcription sequencing, the phylogenetic tree was constructed by ClustalW 1.83 software, Spe I and Xba I restriction sites were introduced to construct over-expression vector pBASTA-CtERF1 containing 35S promoter. Results CtERF1 gene had a functional domain of a typical AP2/ERF gene encoding 297 amino acids, and contained an AP2 region speculated to be located in cytoplasm and nucleus, which was ERF subprotein. Systematic evolution analysis showed that CtERF1 gene had some homology with other plant species, among which the relationship with Populus deltoides and Panax japonicus were the closest. The pBASTA-CtERF1 plant expression vector was constructed successfully by molecular biology. Conclusion A CtERF1 gene of C. tinctorius AP2/ERF transcription factor family was cloned and the plant expression vector pBASTA-CtERF1 was constructed successfully.

国家自然科学基金资助项目（31771868）；国家自然科学基金资助项目（31101172）；国家自然科学基金资助项目（31501366）；吉林省科技厅（20150623024TC-11）；吉林省科技厅（20170520089JH）；吉林农业大学大学生创新创业训练计划项目（2017472）