[关键词]
[摘要]
目的 分析鉴定人参miRNA靶基因。方法 采用降解组测序技术对石柱参(Shizhu ginseng)、园参(Yuan ginseng)的miRNA及靶基因进行检测;利用公共数据库KEGG/NR/GO数据库对降解组基因进行功能注释;通过荧光实时定量PCR(qRT-PCR)技术验证石柱参、园参的miRNA及靶基因表达量。结果 共得到8个miRNA家族的13个靶基因;利用KEGG/NR/GO数据库分析发现该miRNA的靶基因类型主要是转录因子、应答因子及信号转导通路等。对5个差异表达miRNAs:aqc-miR-159、bdi-miR162、cpa-miR319、pgi-miR4376、smo-miR396及其靶基因进行的qRT-PCR验证结果与降解组测序结果一致。结论 明确了部分人参miRNA的靶基因,为进一步研究人参miRNA的可能功能奠定基础。
[Key word]
[Abstract]
Objective To analyze and identify ginseng miRNA. Methods The miRNA and target genes of Shizhu ginseng and Yuan ginseng were detected by the degradation sequencing technology; Functional annotation of Degradome genes was carried out using public databases of KEGG/NR/GO database; The expression of miRNA and target genes of Shizhu ginseng and Yuan ginseng was determinated by real time fluorescence quantitative PCR technique. Results A total of 13 target genes of eight miRNA families were obtained; The target gene type of miRNA was mainly transcriptional factor, response factor, and signal transduction pathway by means of KEGG/NR/GO database analysis. The results of real time fluorescence quantitative PCR verification of aqc-miR-159, bdi-miR162, cpa-miR319, pgi-miR4376, smo-miR396 and its target genes were basically consistent with the expression of miRNA and target genes from the degradation group. Conclusion The target genes of partial Panax ginseng miRNA is clarified, which lays the foundation for further study of the possible function of ginseng miRNA.
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[基金项目]
国家自然科学基金青年基金项目(81403195);广东省自然科学基金项目(S2013010015418)