目的 研究蒲葵子总黄酮（total flavonoids of Fructus Livistonae，TFFL）对对乙酰氨基酚（acetaminophen，APAP）诱导的人正常肝细胞LO₂损伤的作用，初步探究TFFL保护肝损伤的作用机制。方法 MTT法考察TFFL对LO₂细胞的毒性及对APAP诱导的LO₂细胞存活率的影响；流式细胞术和Hoechst 33342染色法考察LO₂细胞凋亡率；试剂盒检测LO₂细胞中丙二醛（MDA）、还原型谷胱甘肽（GSH）水平及天冬氨酸转氨酶（AST）、超氧化物歧化酶（SOD）活性；Western blotting法检测LO₂细胞中诱导型一氧化氮合酶（iNOS）和硝基化酪氨酸微管蛋白（NT）表达水平。结果 TFFL在给定质量浓度下对LO₂细胞无毒性，且可促进其增殖。对APAP诱导的LO₂细胞损伤，TFFL预防给药能够显著减少细胞凋亡，降低细胞中AST活性和MDA水平，升高SOD活性和GSH水平，抑制细胞中iNOS和NT蛋白的表达。结论 TFFL对APAP诱导的LO₂细胞损伤具有一定的保护作用，其作用机制可能与抑制氧化应激和硝化应激有关。

Objective To research the effects of total flavonoids of Livistona chinensis (TFFL) on normal liver LO₂ cells induced by acetaminophen (APAP), and study the liver protective mechanism on liver injury induced by acetaminophen in vitro. Methods The effects of TFFL on LO₂ cells and cell activity induced by acetaminophen were determined by MTT method; The apoptosis rate of LO₂ cells induced by APAP was researched by Flow Cytometry combine with staining agent Hoechst 33342; The levels of malondialdehyde (MDA), aspartate aminotransferase (AST), reduced glutathione (GSH), and superoxide dismutase (SOD) were examined; The expressions of iNOS and nitrotyrosine tubulin (NT) in APAP-induced LO₂ hepatocyte injury cells was studied by Western blotting method. Results TFFL had no toxicity to LO₂ cells at a given concentration which can promote the proliferation. At the same time, experiment results showed that TFFL prophylactically reduced the apoptosis of LO₂ cells induced by APAP. TFFL decreased the level of AST and MDA, increase the level of GSH and SOD, and inhibit the expressions of iNOS protein and NT protein. Conclusion TFFL has a protective effect on LO₂ cells injury induced by APAP. The possible protective mechanism of TFFL is related to the inhibition of oxidative stress and the nitro stress.

广东省重大科技计划项目（2013A022100002）