目的 研究左金方对幽门螺旋杆菌引起人正常胃黏膜上皮（GES-1）细胞增殖与凋亡的影响。方法 幽门螺旋杆菌以不同的感染复数（1：1、50：1、100：l、200：1、300：1）感染GES-1细胞，于12、24、48 h后，CCK-8检测细胞的增殖活性；以不同质量浓度（0.5、1.0、2.0、4.0 μg/mL）左金方作用幽门螺旋杆菌感染的GES-1细胞12、24、48 h后，收集细胞，CCK-8检测细胞的增殖活性，流式细胞仪和Western blotting检测细胞的凋亡率；Hochest染色检测凋亡细胞的形态学改变。结果 幽门螺旋杆菌感染GES-1细胞后，感染复数越大，侵染时间越长，抑制细胞生长和促进细胞凋亡的作用越强，幽门螺旋杆菌以100：1的感染复数作用人GES-1细胞12、24、48 h后，细胞存活率分别降低到（80.57±1.21）%、（70.04±3.21）%、（67.74±2.91）%，细胞的凋亡率分别增加到（23.74±1.71）%、（53.60±1.87）%、（70.67±2.87）%；与1.0 μg/mL左金方共培养24 h后，细胞存活率上升到（97.67±1.04）%，细胞凋亡率降低到（31.04±1.02）%，与相应模型组对比，差异显著（P<0.01）；Western blotting结果表明，左金方各处理组细胞Caspase-3蛋白水平表达均明显降低；Hochest荧光染色，从形态学的角度进一步验证了这一结果。结论 幽门螺旋杆菌感染人GES-1细胞后，可以抑制细胞增殖，诱导细胞凋亡，左金方可以降低幽门螺旋杆菌引起的细胞损伤，保护GES-1细胞。

Objective To study the effect of Zuojin Formula on proliferation and apoptosis of human normal gastric epithelial cells (GES-1) cells infected by Helicobacter pylori. Methods GES-1 cells were infected by H. pylori at different multiplicity of infection (1:1, 50:1, 100:1, 200:1, 300:1) for 12, 24, 48 h; GES-1 cells infected by H. pylori were treated with different concentrations (0.5, 1.0, 2.0, 4.0 μg/mL) of Zuojin Formula, and the cells were harvested after 12, 24, and 48 h. The proliferation activity of cells was detected by CCK-8, and the apoptosis rate of GES-1 cells was measured by Anexin V-FITC apoptosis detecting kit and flow cytometry. Moreover, the expression of Caspase-3 was detected by Western blot and the morphological changes of apoptotic cells were detected by Hochest staining. Results After infected by H. pylori, the cell viability showed a descending trend while the apoptotic rate showed tendency to ascend, with the increasing of multiplicity of infection and infection time. GES-1 cells were treated with multiplicity of infection of H. pylori at 100:1 for 12, 24, 48 h, and the cell viability decreased to (80.57 ±1.21)%, (70.04 ±3.21)%, and (67.74 ±2.91)%, while the apoptotic rate increased to (23.74 ±1.71)%, (53.60 ±1.87)%, and (70.67 ±2.87)%. After co-cultured with 1.0 μg/mL Zuojin Formula for 24 h, the cell viability increased to (97.67 ±1.04)%, and the apoptotic rate decreased to (31.04 ±1.02)%, and the difference was statistically significant compared with the corresponding model group (P < 0.01). The results of Western blotting showed that the protein expression of Caspase-3 of all treatment groups was obviously decreased. Based on the morphological point of view, this result was further verified. Conclusion GES-1 infected by H. pylori NCTC11637 can inhibit cell proliferation and induce apoptosis, while ZuoJinFang could protect GES-1 cells from cell damage.

深圳市知识创新基础研究（自由探索）（JCYJ20170818094033689）；深圳大学教学改革项目（JG2017035）；深圳大学聚徒+创研项目（2018延续）；中药特色技术传承人才培训项目（粤中医办函[2016]54号）