目的 建立黄芩特征化学成分和抗氧化活性相关联的二维指纹图谱，研究黄芩抗氧化活性物质。方法 利用高效液相多检测器联用在线检测体系，对黄芩中化学成分进行检测，共鉴定出抗氧化活性的成分10种；然后采用清除效率为指标对各活性成分的抗氧化活性进行评价。结果 黄芩苷、5，7，2'，5'-四羟基-8，6'-二甲氧基黄酮、5，7，2'-三羟基-6-甲氧基黄酮、黄芩素等化合物具有较强的清除自由基的活性；6-C-阿拉伯糖基-8-C-葡萄糖基白杨素、6-C-葡萄糖基-8-C-阿拉伯糖基白杨素、千层纸素苷、白杨素-7-O-葡萄糖醛酸苷、汉黄芩苷、去甲汉黄芩素等化合物清除自由基的活性次之。结论 采用HPLC-ABTS-DAD-Q-TOF/MS对黄芩中的抗氧化活性成分进行快速分析鉴定，为黄芩的抗氧化物质基础研究提供数据支持。

Objective To establish a two-dimensional fingerprint of Scutellaria baicalensis between characteristic chemical composition and anti-oxidant activity, and to study its anti-oxidant constituents on-line. Methods The scavenging activities of S. baicalensis for ABTS⁺ by HPLC-ABTS-DAD-Q-TOF/MS was studied and its active constituents were screened. A total of ten anti-oxidant components in S. baicalensis were identified. The anti-oxidant activities of different active ingredients were evaluated by anti-oxidant efficiency. Results The data showed that baicalin, 5,7,2',5'-tetrahydroxy-8,6'-dimethoxy flavone, 5,7,2'-trihydroxy-6-methoxyflavone, and baicalein had stronger anti-oxidant activity, and 6-C-arabinosyl-8-C-glucosylchrysin, 6-C-glucosyl-8-C-arabinosyl-chrysin, oroxyloside, chrysin-7-O-glucuronide, wogonoside, and norwogonin had less anti-oxidant activity. Conclusion HPLC-ABTS-DAD-Q-TOF/MS method can rapidly analyze and identify the anti-oxidant components in S. baicalensis, and these experimental data can provide support for the basic research of the anti-oxidant ingredients of S. baicalensis.

国家自然科学基金资助项目（U1504828）；国家自然科学基金资助项目（81603283）；国家自然科学基金资助项目（81603283）；河南省基础与前沿技术研究项目（152300410181）；河南省高等学校重点科研项目（15B360008）；河南省高校青年骨干教师培养计划（2016GGJS-279）；河南省高校科技创新人才支持计划（19HASTIT007）