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[摘要]
目的 对毛茛科铁线莲属植物女萎Clematis apiifolia的化学成分进行研究。方法 通过硅胶、ODS、凝胶Sephadex LH-20、HW-40C等多种色谱分离技术分离和纯化,利用核磁共振(NMR)及质谱等现代波谱学技术鉴定化合物结构。结果 从女萎乙醇提取物中分离得到20个化合物,分别鉴定为常春藤皂苷元(1)、α-hederin(2)、sapindoside B(3)、豆甾-22-烯-3β,6β,9β-三醇(4)、pleuchiol(5)、豆甾醇-3-O-β-D-葡萄糖苷(6)、豆甾醇(7)、毒马草素(8)、莽草酸(9)、乙基-α-D-葡萄糖苷(10)、乙基-β-D-葡萄糖苷(11)、D-葡萄糖醛酸(12)、3,5-二羟基-4-戊内酯(13)、2-醛基-5-羟甲基呋喃(14)、尿嘧啶(15)、尿嘧啶核苷(16)、正二十三烷醇(17)、正三十烷醇(18)、正二十六烷酸(19)、棕榈酸-1-甘油酯(20)。结论 所有化合物均为首次从女萎中分离鉴定,其中化合物3为首次从铁线莲属植物中分离得到,化合物4、5、9~12为首次从毛茛科植物中分离得到。
[Key word]
[Abstract]
Objective To investigate the chemical constituents from the stem of the Clematis apiifolia. Methods A variety of chromatographic methods were applied in isolation and purification including silica gel, ODS, Sephadex LH-20 gel, and HW-40C and so on. Their structures were identified by the nuclear magnetic resonance (NMR), mass spectrometry and so on. Results These compounds were isolated and determined as hederagenin (1), α-hederin (2), sapindoside B (3), stigmast-22-en-3β, 6β, 9β-triol (4), pleuchiol (5), stigmasterol-3-O-β-D-glucopyranoside (6), stimasterol (7), siderin (8), shikimic acid (9), ethyl-α-D-glucopyranoside (10), ethyl-β-D-glucopyranoside (11), D-gulonic acid (12), 3,5-dihydroxyl-4-valerolactone (13), 2-formyl-5-hydroxymethylfuran (14), uracil (15), uridine (16), tricosanol (17), triacontanol (18), hexacosanoic acid (19), and monostearin (20). Conclusion All compounds are obtained from C. apiifolia for the first time including firstly reported compound 3 from Clematis plants and compounds 4, 5, 9 -12 from Ranunculaceae family.
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[基金项目]
江苏省科技基础设施建设计划(BM2014047)