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[摘要]
目的 研究蛇足石杉内生真菌Cercospora lagenariae MT-45的代谢产物。方法 利用硅胶柱色谱及半制备液相色谱等方法进行化合物的分离纯化,并根据化合物的理化性质及MS、NMR数据鉴定其结构;利用体外模型进行抗炎活性筛选。结果 从内生真菌C.lagenariae MT-45的糙米发酵物中分离得到9个聚酮类化合物,分别鉴定为尾孢菌酸A(1)、格链孢酚(2)、交链孢醇单甲醚(3)、(+)-nigrosporaol A(4)、alternarienonic acid B(5)、2-methyl-5-carboxymethyl-7-hydroxychromone(6)、2,5-dimethyl-7-hydroxychromone(7)、1-deoxyrubralactone(8)、(-)-alternarlactam(9)。结论 其中化合物1为新化合物,化合物6对脂多糖(LPS)诱导的小鼠巨噬细胞RAW 264.7一氧化氮(NO)产生具有一定的抑制作用,IC50为(57.5±1.2)μmol/L。
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[Abstract]
Objective To investigate the chemical metabolites from Cercospora lagenariae MT-45, an endophytic fungus isolated from Huperzia serrata (Thunb.) Trev. Methods The compounds were isolated and purified by using silica gel column chromatography and semi-preparative liquid chromatography. The structures were established using physicochemical properties and MS and NMR. The anti-inflammatory activities of all the isolates were also preliminarily investigated by using in vitro model. Results Nine polyketide derivatives including cercolagenlic acid A (1), alternariol (2), alternariol 9-methyl ether (3), (+)-nigrosporaol A (4), alternarienonic acid B (5), 2-methyl-5-carboxymethyl-7-hydroxychromone (6), 2,5-dimethyl-7-hydroxychromone (7), 1-deoxy-rubralactone (8), and (-)-alternarlactam (9) were isolated from C. lagenariae MT-45 fermented on brown rice solids. Conclusion Compound 1 is a new compound, and compound 6 can exhibit a certain inhibition on the nitric oxide production in lipopolysaccharide (LPS)-stimulated RAW 264.7 macrophage cells with an IC50 value of (57.5 ±1.2) μmol/L.
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[基金项目]
国家自然科学基金资助项目(81573312)