[关键词]
[摘要]
目的 建立一种无设备快速30 s提取中药材DNA的方法。方法 使用常规定性滤纸作为核酸吸附的载体,通过裂解液的裂解,释放核酸并吸附在滤纸上,利用洗脱液进行核酸纯化,即可完成不到30 s的整个核酸提取过程,吸附核酸的滤纸可直接放入反应体系进行扩增。结果 此方法可以成功提取不同药用部位的中药材核酸,提取得到的核酸经扩增验证,可获得与传统提取方法相一致的检测结果。结论 此新型快速中药材DNA提取方法简单易行、速度快、成本低,可使核酸提取变得越来越大众化,不再局限于专业人员和实验室环境,为分子生药学的应用和发展提供了新思路。
[Key word]
[Abstract]
Objective To build an equipment-free nucleic acid extraction dipstick methodology that can obtain amplification-ready DNA from Chinese medicinal materials in 30 s. Methods A new rapid DNA extraction method which was suitable for traditional Chinese medicine, with equipment-free nucleic acid extraction dipstick methodology that can obtain amplification-ready DNA from Chinese medicinal materials in 30 s was verified. The filter paper strip or disk for the adsorbed nucleic acid can be directly amplified by the reaction system. Results This method can extract the nucleic acid successfully. The extracted DNA was amplified, and the results were consistent with the traditional extraction methods. Conclusion The new rapid DNA extraction method is simple, fast, and low cost, which will make nucleic acid extraction become more and more popular, and no longer confined to professional and laboratory environment. This study provides a new idea for the application and development of molecular pharmacology.
[中图分类号]
[基金项目]
澳门科技大学中药质量研究国家重点实验室开放课题(MUST-SKL-2016-07);国家中医药管理局全国中药特色技术传承人才培训项目(2015481601003);广州中医药大学2018年校级大学生创新创业训练计划项目(201810572172)