目的 测定金花小檗根、茎和叶及根、茎提取物的总生物碱及其组分含量，研究根、茎提取物及组分对小鼠学习记忆能力和抗氧化能力的改善作用。方法 采用酸性染料比色法测定金花小檗根、茎、叶和根、茎提取物及组分的总生物碱含量，以HPLC法同时测定药根碱、巴马汀和小檗碱的含量。用东莨菪碱制备记忆障碍小鼠模型，ig不同剂量的金花小檗根、茎提取物及组分30 d，以跳台实验和水迷宫实验检测根、茎提取物及组分对小鼠学习记忆的影响，测定血清丙二醛（MDA）和总超氧化物歧化酶（T-SOD）。结果 金花小檗提取物及水溶性组分显著延长小鼠跳台实验的潜伏期（P<0.01），显著减少错误次数（P<0.01）；在一定程度上，缩短水迷宫的定位航行时间（P<0.01），并显著减少错误次数（P<0.05）。根、茎提取物和水溶性组分能显著提高小鼠体内T-SOD活性（P<0.05、0.01），而显著降低MDA水平（P<0.05、0.01）。结论 金花小檗根、茎提取物及水溶性组分对小鼠的学习记忆能力有一定的改善作用，对其体内抗氧化能力也有提高。

Objective To determine the content of total alkaloids and their components in roots, stems, and leaves of Berberis wilsonae, and investigate the effects of their extracts and components on the improvement of learning and memory ability and anti-oxidant activity of mice. Methods In this study, the total alkaloid content of roots, stems, leaves, and roots, stem extracts and components of B. wilsonae was determined by acid dye colorimetry. The HPLC method and conditions were established for determination jateorhizine, palmatine, and berberine. The memory impairment mice model was established with scopolamine, and the root and stem extracts and components of B. wilsonae were ig administrated for 30 d. Jumping platform test and water maze test were used to detect the effect of roots and stem extracts and components on learning and memory, and the levels of MDA and T-SOD in serum was determinated by kit method. Results The results showed that the latency of the mice in step-down test was significantly prolonged (P < 0.01), and the reduction of the number of errors was also significant (P < 0.01). To a certain extent, the place navigation time in water maze was shortened (P < 0.01), and the number of errors was reduced significantly (P < 0.05). Extracts and components can significantly increase the level of T-SOD (P < 0.05, 0.01) in mice, and reduce the level of MDA significantly (P < 0.05, 0.01). Conclusion The extracts and components of B. wilsonae had a certain effect on the improvement of learning and memory ability of mice, and the anti-oxidant capacity in vivo was also improved.

四川省教育厅重点项目（17ZA0328）；西昌市教育和科学技术局2014年产学研合作项目；四川省教育厅项目（17ZB0353）