目的 采用超滤亲和-液质联用（Ultra-filtration affinity-liquid chromatography-mass spectrometry，UF-LC-MS）和分子对接技术筛选毛菊苣Cichorium glandulosum根中α-葡萄糖苷酶抑制剂。方法 通过超滤亲和-液质联用技术筛选并鉴定毛菊苣根中的α-葡萄糖苷酶抑制剂，发现有4个组分对α-葡萄糖苷酶的活性具有抑制作用，进一步采用分子对接技术对这4个化合物进行验证。结果 发现2个化合物对α-葡萄糖苷酶具有较高的抑制作用，其中黄芩苷、山莴苣苦素在0.1~2.0 mg/mL呈良好的量效关系。结论 为利用UF-LC-MS和分子对接技术筛选天然产物中α-葡萄糖苷酶抑制剂提供了参考，也为后续研究毛菊苣降糖功效提供了基础。

Objective To screen α-glucosidase inhibitors from root extract of Cichorium glundulosum (CGR) by UF-LC-MS and molecular docking. Methods The inhibitory activity of CGR extract was evaluated by establishing enzyme inhibitor model, Meanwhile, four active compounds from CGR extract were screened by UF-LC-MS and identified as α-glucosidase inhibitors, which were verified by using Autodock Software. Results The results showed that two compounds that combined with α-glucosidase well were screened out, including baicalin and lactupicrin, which showed significant α-glucosidase inhibitory activity in the concentration range of 0.1-2 mg/mL in a dose-dependent manner. Conclusion Our work provide theoretical basis for screening natural and high affinity enzyme inhibitors from CGR by using UF-LC-MS and molecular docking and further study of the hypoglycemic effect of C. glundulosum.