[关键词]
[摘要]
目的 基于质量标志物(Q-marker)理念,从化学成分的角度对多基原藏药材"美多罗米"的质量标志物进行预测,评价其药材品质。方法 首先基于亲缘关系与生源途径、药理作用与化学成分的关系,预测美多罗米的Q-marker;其次采用UHPLC同时测定其主要活性成分,采用Acquity UHPLC HSS C18色谱柱(100 mm×2.1 mm,1.8 μm);流动相为乙腈(A)-0.3%乙酸水(B),梯度洗脱,体积流量为0.21 mL/min,柱温35℃,检测波长为320 nm。结果 预测咖啡酸类成分(绿原酸、咖啡酸、3,4-二咖啡酰奎宁酸、3,5-二咖啡酰奎宁酸、4,5-二咖啡酰奎宁酸)是美多罗米的主要活性物质基础之一,可作为Q-marker的主要选择。建立了美多罗米中咖啡酸类成分的含量测定方法,绿原酸、咖啡酸、3,4-二咖啡酰奎宁酸、3,5-二咖啡酰奎宁酸、4,5-二咖啡酰奎宁酸分别在9.60~480.00、10.27~513.50、10.08~504.03、9.64~482.80、3.82~380.24 mg/L线性关系良好,平均加样回收率分别为102.43%、99.78%、99.39%、103.12%、101.83%。各样品中均能检测出这5个成分,总含量在全草中均大于10%,提示这5个咖啡酸类成分可以作为美多罗米的Q-marker。结论 以咖啡酸类成分作为美多罗米的Q-marker科学合理;建立的美多罗米多基原植物中咖啡酸类成分的UHPLC测定方法可用于美多罗米药材质量控制,为多基原药材合理评价提供新思路。
[Key word]
[Abstract]
Objective Based on the concept of quality marker (Q-marker), a chemical method was developed to evaluate the quality of the multi-original medicinal material "Meiduoluomi" and predict its Q-marker. Methods Firstly, the Q-marker were preliminary predicted based on the relationship between genetic relationship and biosynthesis pathway, pharmacological activity and chemical composition. Secondly, the main active components were determined by UHPLC. The analysis was performed using ACQUITY UHPLC HSS C18 column (100 mm×2.1 mm, 1.8 μm); Mobile phase was acetonitrile (A)-0.3% acetic acid water (B) with gradient elution; The flow rate was 0.2 mL/min; The column temperature was 35℃; The detection wavelength was 320 nm. Results A new method for simultaneous determining five compounds of meiduoluomi was established, including chlorogenic acid, caffeic acid, 3,4-dicaffeoylquinic acid, 3,5-dicaffeoylquinic acid, and 4,5-dicaffeoylquinic acid. The linear relationship of them was good at the range of 9.60-480.00, 10.27-513.50, 10.08-504.03, 9.64-482.80, and 3.82-380.24 mg/L, respectively; And the average recovery rates were 102.43%, 99.78%, 99.39%, 103.12%, and 101.83%, respectively. The five components were detected in each sample and the total content of the five components was more than 10% in the herbal medicine. It was suggested that the five components can be used as a Q-marker of meiduoluomi for quality control. Conclusion The coffeic acid components are scientific and reasonable to be considered as a Q-marker of meiduoluomi. The developed UHPLC method can be used for the quality control of meiduoluomi. This study provides new idea for the rational evaluation of multi-original medicinal material based on Q-marker.
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[基金项目]
国家自然科学基金项目(31870314);四川省中医药管理局项目(2018JC0027);西南民族大学研究生创新型科研项目(CX2019SZ167)