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[摘要]
目的 考察黄连濒危物种雅连Coptis deltoidea在传统加工前后,对2型糖尿病大鼠血糖、血脂的作用差异。方法 采用高糖、高脂饮食复合小剂量链脲佐菌素(STZ)诱导的方法复制大鼠2型糖尿病模型,考察雅连分别采用黄酒、生姜和吴茱萸炮制后,与生品相比,对模型大鼠空腹血糖(FBG)、空腹血清胰岛素(FINS)、糖化血清蛋白(GSP)、血清三酰甘油(TG)、总胆固醇(TC)、脂蛋白(HDL-C/LDL-C)水平,以及SCAP/SREBP-1c固醇调节通路关键蛋白及其基因表达的药效及差异。结果 在降糖方面,雅连及其炮制品均能降低模型大鼠FBG及GSP水平,修复受损胰腺,但雅连经黄酒和吴茱萸炮制后,对FBG及GSP降低作用更为显著(P<0.01),其中以酒炙品降糖幅度最大;在调脂方面,雅连及其炮制品均能够降低模型大鼠血清TG、TC、LDL-C水平(P<0.05、0.01),提高HDL-C水平(P<0.05、0.01),并下调胰岛素靶器官肝脏SCAP、SREBP-1c蛋白及其基因表达(P<0.05、0.01),但酒炙及萸炙品优于姜炙品或雅连生品。结论 用黄酒、生姜、吴茱萸对雅连进行炮制均为改善雅连本性苦寒不易久服弊端的传统方法,但不同炮制方式对雅连药效有不同影响,临床用药时应辨证施治,单纯降糖选用雅连酒炙品为宜;而降糖调脂综合药效则以酒炙及萸炙品更优。
[Key word]
[Abstract]
Objective To investigate the difference of hypoglycemic and hypolipidemic effects of Coptis deltoidea (Coptis chinensis) and its processed products on T2DM rats. Methods Type 2 diabetes mellitus induced by high sugar, high fat diet and low dose of streptozotocin. C. deltoidea were processed with yellow wine, ginger, and evodia rutaecarpa, respectively. The effects of C. deltoidea on fasting blood glucose (FBG), fasting insulin (FINS), glycosylated serum protein (GSP), pancreatic pathology, serum triglyceride (TG), total cholesterol (TC) and lipoprotein (HDL-C or LDL-C) and key protein and gene expression of SCAP\SREBP-1c pathway in liver of model rats were investigated.Result C. deltoidea and its processed products reduced the FBG and GSP in T2DM rats. The wine products significantly reduced FBG and GSP in model rats (P < 0.01). The hypoglycemic range was better than raw and ginger products. At hypolipidemic aspect, C. deltoidea and its processed products decreased the content of TG, TC, and LDL-C (P < 0.05, 0.01), increased the content of HDL-C in serum (P < 0.05, 0.01), and down-regulated the protein and gene expression of SCAP/SREBP-1c in liver, the insulin target organ. However, wine and evodia products were better than crude drug or ginger products.Conclusion C. deltoidea processed by yellow wine, ginger and evodia rutaecarpa is traditional method to improve the disadvantages of C. deltoidea with bitter cold nature and not easy to take for a long time. However, different processing methods have different effects. Clinical medication should be combied withTCM syndrome differentiation and simple hypoglycemic effect of wine product is appropriate; while wine and evodia products were better in hypoglycemic and lipid regulation aspects.
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[基金项目]
国家自然科学基金青年基金项目“藏医名方中有效单体挖掘及其诱导EPCs调控脑缺血后血管再生修复的研究”(81703813);国家自然科学基金面上项目“藏医抗脑缺血名方药效物质的神经保护机制研究”(81473337);国家发改委/国家中医药管理局联合中药标准化行动专项“中药材饮片黄柏标准化建设”(2YB2H-Y-SC-41);四川省中医药管理局重点项目“川产道地药材产业化发展示范县项目-黄连”(2016Z008);国家重大专项“葛黄有效组分防治2型糖尿病候选药物研究”(2013ZX09103-002-014);四川中医药资源普查成果转化项目“川黄连炮制品的降糖制剂产品开发研究”(2016z014);四川省中医药管理局2016科技项目“姜黄素新型脑靶向制剂制备及评价”(2016Q038);四川省中医药管理局2017科技项目“红景天美白会呼吸面膜的研制”(2017C001)