[关键词]
[摘要]
目的 研究宽叶打碗花Calystegia sepium中的化学成分。方法 利用硅胶柱色谱、凝胶柱色谱、反相柱色谱和制备液相色谱等方法进行分离纯化,通过理化性质、波谱数据并结合文献报道数据鉴定化合物结构。结果 从宽叶打碗花中分离得到20个化合物,分别鉴定为酪醇(1)、槲皮素-3-O-β-D-吡喃葡糖苷(2)、野黄芩素-7-O-β-吡喃葡萄糖苷(3)、紫云英苷(4)、山柰酚-3-O-半乳糖苷(5)、山柰酚-3-O-β-刺槐双糖苷(6)、烟花苷(7)、芦丁(8)、尿苷(9)、它乔糖苷(10)、2-甲氧基-4-(2'-羟乙基)-苯基-1-O-β-D-吡喃葡萄糖苷(11)、4-羟基苯乙醇-4-O-β-D-吡喃葡萄糖苷(12)、polybotrin(13)、吡啶-3,5-二甲酰胺(14)、茵芋苷(15)、苯甲醇-β-D-葡萄糖苷(16)、5-羟基-2-羟甲基吡啶(17)、云杉苷(18)、5-羟甲基糠醛(19)、sessiline(20)。结论 所有化合物均为首次从该植物中分离得到。
[Key word]
[Abstract]
Objective To study the chemical constituents from Calystegia sepium.Methods The compounds were isolated and purified by silica gel column chromatography, Sephadex LH-20, ODS column chromatography, preparative HPLC and so on. Their structures were determined on the basis of physicochemical properties and their spectroscopic data, as well as literatures.Results A total of 20 compounds were separated and identified as tyrosol (1), quercetin-3-O-β-D-glucopyranoside (2), scutellarein-7-O-β-glucopyranoside (3), kaempferol-3-O-β-D-glucopyranoside (4), kaempferol-3-O-galactoside (5), kaempferol-3-O-β-robinobioside (6), nicotiflorin (7), rutin (8), uridine (9), tachioside (10), 2-methoxy-4-(2'-hydroxyethyl)-phenyl-1-O-β-D-glucopyranoside (11), 4-hydroxyphenethylol-4-O-β-D-glucopyranoside (12), polybotrin (13), pyridine-3,5-dicarboamide (14), skimmin (15), benzylalcohol-β-D-glucopyranoside (16), 5-hydroxyl-2-hydroxymethylpyridine (17), picein (18), 5-hydroxymethyl-furaldehyde (19), and sessiline (20).Conclusion Compounds 1-20 are isolated from Calystegia sepium for the first time.
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[基金项目]
国家自然科学基金资助项目(81202885);广东省自然科学基金资助项目(2015A030313585)