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[摘要]
目的 研究剑叶耳草Hedyotis caudatifolia的化学成分。方法 采用硅胶、聚酰胺、Sephadex LH-20凝胶等柱色谱技术进行分离纯化,通过化合物的核磁共振、质谱等光谱数据和理化性质鉴定其结构。结果 从剑叶耳草的甲醇提取物中分离得到10个化合物,其结构分别鉴定为剑叶耳草苷A(1)、β-谷甾醇(2)、5α-豆甾烷-3,6-二酮(3)、7-羟基-6-甲氧基香豆素(4)、齐墩果酸(5)、乌苏酸(6)、坡模酸(7)、3α,19α-二羟基-12-烯-24,28-乌苏酸(8)、2α,3β,24-三羟基-12-烯-28-齐墩果酸(9)、2α,3α-二羟基-12-烯-28-齐墩果酸(10)。结论 化合物1为新的咖啡酸葡萄糖苷,化合物7~10均为首次从该植物中分离得到。化合物1具有一定的抑制肿瘤细胞生长活性。
[Key word]
[Abstract]
Objective To study the chemical constituents of Hedyotis caudatifolia.Methods These compounds were isolated from H. caudatifolia and purified by repeated silica gel, polyamine, Sephadex LH-20 column chromatography and other methods. Their structures were identified by NMR, MS, and physico-chemical properties.Results Ten compounds were isolated and elucidated as hedycaffeoylglucoside A (1), β-sitosterol (2), 5α-stigmastane-3,6-dione (3), 7-hydroxy-6-methoxycoumarin (4), oleanolic acid (5), ursolic acid (6), pomolic acid (7), 3α,19α-dihydroxyurs-12-en-24,28-dioic acid (8), 2α,3β,24-trihydroxyolean-12-en-28-oic acid (9), and 2α,3α-dihydroxyolean-12-en-28-oic acid (10), respectively.Conclusion Compound 1 is a new caffeoyl glucoside and compounds 7-10 are isolated from this plant for the first time. Compound 1 exhibits cytotoxicity against four tumor cells.
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[基金项目]
国家自然科学基金面上项目(31170701)