目的 预测磷脂酰肌醇4-磷酸5-激酶（PIP5Ks）基因家族7个基因的表达部位；测定Lm型雌性系蓖麻aLmAB2 3种品系不同时期的蓖麻花序中PIP5Ks各成员相对表达量。方法 利用生物信息学分析在线软件进行蛋白质理化性质分析、蛋白质疏水性分析、亚细胞定位预测、跨膜区分析。采用荧光定量PCR方法进行蓖麻PIP5Ks基因家族的生物信息学分析和采用DNAMAN程序进行序列比对。结果 蓖麻PIP5Ks共有7个成员，分别为PIP5K1、PIP5K2、PIP5K4、PIP5K6、PIP5K8、PIP5K9、PIP5K11；蓖麻PIP5K对应的蛋白之间的氨基酸序列同源性达到48.06%，蓖麻PIP5Ks所对应蛋白均为亲水性蛋白，除PIP5K9和PIP5K11为不稳定蛋白质其余均为稳定蛋白质；PIP5Ks对应蛋白均不存在跨膜结构域，均为非跨膜蛋白。亚细胞定位结果显示PIP5K1、PIP5K4、PIP5K6、PIP5K9、PIP5K11蛋白具有导肽的可能性较低，并且无对应的氨基酸切割位点，即定位在其他细胞器或者可能为胞浆蛋白；PIP5K2蛋白定位在叶绿体，叶绿体转运肽数值较高；PIP5K8蛋白定位在分泌通路。利从PIP5Ks在差异表达的相对表达量变化上来看，除PIP5K4并未检测到荧光，其余PIP5Ks均有一定的差异表达，PIP5Ks的相对表达量的变化趋势均较为相近，PIP5K1、PIP5K2的相对表达量较多，其次为PIP5K8、PIP5K9的相对表达量，PIP5K6及PIP5K11只有少量的表达。结论 PIP5Ks在蓖麻中对花序轴性状可能有一定的影响，PIP5Ks在花序轴上的差异含量与花序发育具有一定的规律性。

Objective To predict the expression of seven genes in the PIP5K gene family, and to determine the relative expression of PIP5Ks in different stages of type Lm gynoecious Ricinus communis aLmAB2. Method Bioinformatics analysis of online software protein physicochemical properties analysis, protein hydrophobicity analysis subcellular localization prediction, transmembrane region analysis, etc. Bioinformatics analysis of hemp PIP5K gene family and DNAMAN for sequence alignment and real-time PCR. Results The results showed that there were seven members in the R. communis PIP5Ks, which were PIP5K1, PIP5K2, PIP5K4, PIP5K6, PIP5K8, PIP5K9, and PIP5K11. The amino acid sequence homology of the corresponding protein of R. communis PIP5K reached 48.06%, and the protein of R. communis PIP5Ks all are hydrophilic proteins. Except that PIP5K9 and PIP5K11 were unstable proteins, the rest were stable proteins, and the corresponding proteins of PIP5Ks have no transmembrane domain, and all were non-transmembrane proteins. Subcellular localization results showed that the proteins PIP5K1, PIP5K4, PIP5K6, PIP5K9, and PIP5K11 were less likely to have a lead peptide, and there was no corresponding amino acid cleavage site, ie, localized in other organelles or possibly cytoplasmic proteins; Protein PIP5K2 was localized in chloroplasts, the chloroplast transit peptide has a higher value, and the protein PIP5K8 was located in the secretory pathway. From the perspective of the relative expression of PIP5Ks in the differential expression, except that no fluorescence was detected in PIP5K4, the other PIP5Ks had a certain differential expression. The changes of relative expression of PIP5Ks showed similar trends, the levels of PIP5K1, PIP5K2 relative expression were high, followed by the relative expression of PIP5K8, PIP5K9, and PIP5K6 and PIP5K11 only have fewer expressions. Conclusion It is concluded that PIP5Ks may have a certain effect on the inflorescence axis traits in ramie. The differential expression of PIP5K on the inflorescence axis has the certain regularity with the development of inflorescence.

国家自然科学基金项目（31160290）；国家自然科学基金项目（31860353）；国家自然科学基金项目（31860071）；国家青年科学基金项目（31401418）；内蒙古自治区自然科学基金面上项目（2017MS0339）；内蒙古自治区科技创新引导奖励资金项目（KJCX15002）；内蒙古自治区“草原英才”计划项目（201511）；内蒙古自治区草原英才创新团队——蓖麻分子育种创新研究团队支持项目（2017）；内蒙古自治区蓖麻育种重点实验室开放基金项目（MDK2016031，MDK2016030）；内蒙古自治区高校蓖麻产业工程技术研究中心开放基金项目（MDK2017030，MDK2018014，BMYJ2015-07，MDK2016008）；内蒙古民族大学研究生科研项目（NMDSS1863）；内蒙古民族大学2017研究生重点建设课程项目（生物工程技术原理）；内蒙古民族大学2018年研究生重点建设课程项目（现代分子生物学新技术）