目的 利用网络药理学技术预测黄连解毒汤治疗高血压的作用机制。方法 从中药系统药理学分析平台（TCMSP）中寻找与黄连解毒汤中4味中药相关的所有化学成分和作用靶点，构建化合物靶点相互作用网络图；通过OMIM数据库、TTD数据库以及PharmGkb数据库筛选高血压疾病相关的靶标；PPI数据库构建黄连解毒汤和高血压的交互靶标；筛选药物靶点和疾病靶点相互作用的核心靶点，采用Davidv 6.8数据库对核心靶点进行Gene Ontology（GO）分析，利用KEGG数据库对核心靶点进行相关通路富集。结果 选择口服利用度（OB） ≥ 30%、类药性（DL） ≥ 0.18作为化合物分子的筛选条件，并通过文献查找加以补充，共筛选出黄连解毒汤的61个潜在活性成分和154个潜在靶点；通过度、介度中心度、接近中心度等网络拓扑特征评价筛选出与黄连解毒汤在高血压方面作用核心靶点228个，GO分析共包含118条富集结果，其中生物过程59条，分子功能31条，细胞组成28条。利用KEGG数据库对入选靶标进行相关通路富集，筛选出45条通路在高血压疾病方面具有作用。结论 黄连解毒汤具有多成分、多途径、多靶点协同作用的作用特点，预测了黄连解毒汤治疗高血压疾病的可能作用机制，为寻找其有效成分和作用机制及临床应用奠定基础。

Objective To study the mechanism of Huanglian Jiedu Decoction (HJD) in the treatment of hypertension by using network pharmacology. Methods Chemical components and targets related to the four herbs of Chinese meteria medica in HJD were searched through the traditional Chinese medicine systems pharmacology database and analysis platform (TCMSP) to construct the interaction network diagram of the target point of the compounds. The hypertension related targets were screened through OMIM, TTD, and PharmGkb databases; The interactive target of HJD and hypertension was constructed in PPI database; GO analysis of screened core targets between drug target and disease target was carried out using Davidv 6.8 database, and the related pathways of core targets were enriched using KEGG database. Results Using the oral bioavailability (OB) ≥ 30% and drug likeness (DL) ≥ 0.18 as screening condition for the compounds, combined with supplement literatures searched, 61 active components and 154 corresponding protein targets of HJD were screened out. A total of 118 enrichment results were obtained from 228 core targets of HJD in hypertension, including 59 biological processes, 31 molecular functions, and 28 cell compositions. The selected targets were enriched by KEGG database and 45 pathways playing an important role in hypertension were screen out. Conclusion The synergetic effect of HJD with multi-components and multi-pathway was confirmed by network pharmacology, and the main possible mechanism of HJD in treating hypertension was predicted, which lay a foundation for the identification of effective components, the mechanism of action, and clinical application.

第二批国家“万人计划”百千万工程领军人才项目资助