目的 比较熟地黄不同炮制品对苦寒泻下合并劳倦过度型脾虚大鼠胃肠运动的影响。方法 取80只SD大鼠，按体质量随机分成8组，即对照组、模型组、九蒸九晒熟地黄组（熟地黄Ⅰ组）、九蒸九晒（缺黄酒）熟地黄组（熟地黄Ⅱ组）、九蒸九晒（缺砂仁）熟地黄组（熟地黄Ⅲ组）、九蒸九晒（缺黄酒和砂仁）熟地黄组（熟地黄Ⅳ组）、清蒸熟地黄组（熟地黄Ⅴ组）、酒炖熟地黄组（熟地黄Ⅵ组）。通过对大鼠ig大黄水煎液，同时实施游泳2~5 min，以其身体下沉为度，每日1次，获得苦寒泻下合并劳倦过度型脾虚模型后，分别ig给予熟地黄Ⅰ~Ⅵ组供试品溶液，通过观察脾虚大鼠的一般体征，测定脾虚大鼠血清D-木糖、胃泌素（GAS）、胃动素（MTL）含量，观察熟地黄不同炮制品对脾虚大鼠胃肠消化吸收能力及肠道推进运动的影响。结果 针对苦寒泻下合并劳倦过度脾虚模型，九蒸九晒熟地黄ig给药后较造模后大鼠体质量、进食量与进水量有明显的增加（P<0.01），而清蒸熟地黄、酒炖熟地黄ig给药后较造模后大鼠体质量、进食量与进水量有所减少（P<0.05）。熟地黄Ⅲ、Ⅳ、Ⅴ、Ⅵ组大鼠的肠道推进率、血清D-木糖、GAS和MTL含量低于对照组（P<0.05、0.01）；熟地黄Ⅳ、Ⅴ、Ⅵ组大鼠的肠道推进率、血清D-木糖含量低于熟地黄Ⅰ组（P<0.05、0.01），熟地黄Ⅳ组大鼠的血清GAS和MTL含量低于熟地黄Ⅰ组（P<0.01）。结论 九蒸九晒炮制方法有效地缓解熟地黄质厚味浓，滋腻碍脾的弊端。

Objective To compare the effects of prepared Rehmanniae Radix Praparata (RRP) on gastrointestinal motility in rats with purgation with bitter cold and excessive fatigue type spleen deficiency. Methods A total of 80 SD rats were randomly divided into eight groups according to their body weights, which named as control group, model group, nine steamed nine sunned RRP group (RRP Ⅰ), nine steamed nine sunned (without yellow wine) RRP group (RRP Ⅱ group), nine steamed nine sunned (without Amomi Fructus) RRP group (RRP Ⅲ group), nine steamed nine sunned (without yellow wine and Amomi Fructus) RRP group (RRP IV group), steamed RRP group (RRP V group), and wine stewed RRP group (VI group). By filling the rats with Rhei Radix et Rhizoma decoction and swimming 2 to 5 min at the same time, with its body sinking as degree, once daily, after obtaining the model of purgation with bitter cold and excessive fatigue type spleen deficiency of the RRP groups I-VI, the spleen deficiency rats' general physical signs were observed, and the serum D-xylose, GAS, and MTL of the spleen asthenia rats were measured. The effects of different processed products of RRP on gastrointestinal digestion and absorption and intestinal propulsion in rats with spleen deficiency were observed. Results The weight, food intake, and influent amount of model rats were significantly increased after nine steamed and nine sunned RRP treatment (P < 0.01), while the weight, food intake, and water intake of the rats in RRP V and VI groups after administration were decreased compared with the model rats (P < 0.05). By measuring the intestinal propulsion rate, D-xylose, GAS, and MTL content in the serum, the intestinal propulsive rate, the serum D-xylose, GAS, and MTL content of the rats in RRP Ⅲ, IV, V, and Ⅵ groups were lower than those of the control group (P < 0.01). The intestinal propulsive rate and the content of serum D-xylose in the RRP V group and RRP VI group were lower than that in the RRP Ⅰ group (P < 0.01). The intestinal propulsive rate and the serum D-xylose content in rats of RRP IV group were lower than those in the RRP Ⅰ group (P < 0.05). The serum GAS and MTL content in rats of RRP IV group were lower than those in the RRP Ⅰ group (P < 0.01). Conclusion Nine steamed nine sunned processing method can effectively alleviate the disadvantages of RRP because of its strong flavor and rich spleen.

国家自然科学基金面上项目（81372899）；安徽省高校自然科学研究重点项目（KJ2018A0983）